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Updated: Jan 15, 2026

TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

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单分子色氨酸分析揭示了细胞类型特定的A/B区间改变和多增强剂转录协调

Luo-Ran Liu1, Jia-Yong Zhong2, Xin Bai1

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, Guangdong 510060, China.

Journal of genetics and genomics = Yi chuan xue bao
|October 10, 2025
PubMed
概括

我们开发了nanoCAM-seq,这是一种新的测序方法,可以同时分析染色质相互作用,可访问性和CpG甲基化. 这项技术揭示了这些表观遗传特征如何在A/B区间过渡期间协调基因调节.

关键词:
三维基因组是3D的染色体的可访问性 染色体的可访问性通过DNA甲基化.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.基因调节 基因调节

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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations11:36

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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
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Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
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