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一个中性多价值的AIE-TICT支架:一种关闭的,无需洗的化策略,用于生物感应活细胞和真菌细菌中的甘氨酸-蛋白相互作用

Huai-En Chang¹, Chien-Chin Chen², Yi-Jin You¹

¹Department of Chemistry and Biochemistry, National Chung Cheng University, Minhsiung, Chiayi, 621, Taiwan.

Analytica chimica acta

|October 10, 2025

概括

研究人员开发了一种新的光探针,即粘度敏感的TPE-朱洛利丁 (VTJ) 支架,用于无洗的细胞相互作用成像. 这种探针提供了增强的灵敏度和选择性,可以在没有洗步骤的情况下检测癌细胞和真菌菌.

科学领域:

生物化学生物化学
分子成像学分子成像学
化学生物学化学生物学

背景情况:

目前用于成像弱相互作用的光探测器,如甘氨酸-甘氨酸结合蛋白 (GBP) 识别,反应不佳和背景高.
需要一个模块化,坚固和选择性的平台,用于无洗成像.

研究的目的:

开发一种新的化基架,用于对生物相互作用进行选择性成像.
创建一个平台,使特定细胞点的无洗,增强的光检测.

主要方法:

设计了一种四乙烯 (TPE) - 朱洛利丁框架 (VTJ),利用聚合诱导的排放和扭曲的分子内电荷转移.
合成和测试了与糖甘结合的VTJ探针,用于选择性成像癌细胞和真菌菌.
使用二甲基甘氨酸探针,通过多价值来增强信号放大.

主要成果:

VTJ探针在目标结合时显示出十倍的"关闭-启动"光增强,限制了分子内运动.
VTJ-Di-Galactose和VTJ-Di-N-乙烯基神经胺酸分别被选择性标记为HepG2和PANC-1癌细胞.
VTJ-Mono-Trehalose被内化到Mycobacterium smegmatis中,并在细胞壁结合时进行光,在活细胞中观察到度依赖的颜色变化.

结论:

VTJ支架是第一个通过糖结合在生物系统中进行选择性标记的化系统.
它的模块化,光稳定性和可调色性质为各种成像应用提供了生物结合.
VTJ探针显示了与动态矩阵集成的潜力,并提供了关于癌细胞迁移和转移的见解.

关键词:

聚合引起的排放 (AIE)细菌的新陈代谢发生在细菌中.癌细胞检测癌细胞检测化物探测器的探测器是化物.葡萄糖蛋白相互作用扭曲的分子内电荷转移 (TICT)

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