HAND2侵入了核凝聚物,开创了特定于血统的心脏起器基因程序
Kartik Kulkarni1, Gabriella Quinn2, Jared Dela Rosa1
1Department of Internal Medicine, Cardiology Division, University of Texas Southwestern Medical Center, Dallas, TX, USA.
核子对核糖体组装至关重要,也调节了基因表达. 像HAND2这样的转录因子定位到核细胞中,以激活压抑的异染色体内心脏起器基因.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 核,传统上被称为核糖体生物发生,也组织转录抑制的异色素蛋白成核相关域 (NAD).
- NADs含有对细胞类型规范至关重要的基因,但转录因子 (TF) 接入和基因激活的机制在这个异染色体内尚不清楚.
研究的目的:
- 研究细胞类型重编程过程中核在调节基因表达中的作用.
- 阐明转录因子在核细胞内获取异染色素以激活谱系特异性基因的机制.
主要方法:
- 利用TF诱导的心脏起器重编程的模型.
- 进行了公正的转录分析,以分析基因表达模式.
- 研究了转录因子HAND2.2的核细胞定位和功能.
主要成果:
- 证明HAND2的核细胞局部化对于心脏起器血统转换至关重要.
- 揭示了心脏起器基因程序在细胞核内被细分为部分.
- 显示HAND2同质体进入核凝聚物并结合特定的DNA动机以激活NADs内的增强剂.
结论:
- 细胞核在调节由HAND2.2介导的起器基因表达方面发挥着至关重要的作用.
- TF局部化到亚核异色素蛋白域,如核细胞,是激活谱系特定基因程序的重要机制.
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