Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.6K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

6.5K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.5K
Actin Polymerization01:42

Actin Polymerization

8.3K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.3K
Proteoglycans01:05

Proteoglycans

4.7K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
4.7K
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

2.4K
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
2.4K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

3.1K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

De novo design of RNA pseudoknots with deep learning.

bioRxiv : the preprint server for biology·2026
Same author

Cytoplasmic region of beta-dystroglycan is essential for postsynaptic maturation and neuromuscular function in mice.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Molecular architecture of the fungal-specific potassium channel TOK1.

Nature communications·2026
Same author

Resolving heterogeneity of targeted lipid nanoparticles through solution-based biophysical analyses.

bioRxiv : the preprint server for biology·2026
Same author

Targeting Circulating FABP4 Ameliorates Obesity-Associated Hepatic Steatosis.

bioRxiv : the preprint server for biology·2026
Same author

Multiscale structure of chromatin condensates explains phase separation and material properties.

Science (New York, N.Y.)·2025

相关实验视频

Updated: Jan 15, 2026

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
12:54

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

12.9K

在LARGE1过程中聚合了长度控制的母糖甘在prodystroglycan上.

Soumya Joseph1,2,3, Nicholas J Schnicker2,4, Nicholas Spellmon5

  • 1Senator Paul D. Wellstone Muscular Dystrophy Specialized Research Center, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA, USA.

Nature communications
|October 10, 2025
PubMed
概括

通过LARGE1合成母糖需要特定的蛋白质域和原料. 这种酶过程性聚合matriglycan,其长度由dystroglycan产域控制,提供治疗潜力.

更多相关视频

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.3K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

7.1K

相关实验视频

Last Updated: Jan 15, 2026

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
12:54

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

12.9K
Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.3K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

7.1K

科学领域:

  • 生物化学 生物化学
  • 葡萄糖生物学 葡萄糖生物学
  • 分子生物学分子生物学

背景情况:

  • 马特里格利干是一种关键的细胞外矩阵甘氨酸,对神经肌肉功能至关重要.
  • 孕糖合成中的缺陷导致肌肉发育不正常和发育异常.
  • LARGE1 (Like-acetylglucosaminyltransferase-1) 是一个独特的酶,它负责在dystroglycan上对matriglycan的合成.

研究的目的:

  • 通过LARGE1.1阐明基甘聚合的机制.
  • 确定LARGE1活动的基本组成部分和监管因素.
  • 为治疗针对母糖缺乏症的治疗策略提供基础.

主要方法:

  • 在实验室中,使用复合性prodystroglycan.LARGE1活性的复制.
  • 使用LARGE1活性位点的位点导向突变发生的酶分析.
  • 对马特里格利干聚合物的要求的描述,包括蛋白质域和原料的存在.

主要成果:

  • 由LARGE1进行的母糖聚合需要二糖N终端域 (DGN),酸化核心M3和酸糖原料.
  • LARGE1 呈现出在产甘上过程性聚合活性.
  • 合成的母糖甘的长度由双糖甘原域 (DGN) 调节.

结论:

  • 这项研究揭示了由LARGE1.1进行的母糖甘合成的详细机制.
  • 了解这种机制对于开发对与母糖相关的神经肌肉疾病的疗法至关重要.
  • 这项研究也对理解拉萨热病病毒相互作用有影响.