Hirudinaria manillensis的完整的cox 1基因和ITS序列以及分子进化分析
Wenyi Wang1, Chaojie Yang1, Guangzong He1
1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China.
Scientific reports
|October 10, 2025
概括
使用cox 1基因对hirudinaria manillensis的分子鉴定揭示了中国南部人口数量的增加. 尽管有这种增长,但稳定的遗传多样性尚未形成,这表明最近由于人工种植而发生的迁移和繁殖.
科学领域:
- 动物学 动物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- Hirudinaria manillensis是一种药用吸血虫,对于治疗心血管和脑血管疾病至关重要.
- 分子鉴定为遗传进化分析提供准确和稳定的生物信息.
研究的目的:
- 通过使用完整的cox 1基因序列来分子识别Hirudinaria manillensis.
- 分析中国南部Hirudinaria manillensis的遗传多样性和种群结构.
主要方法:
- 为完整的细胞染色体c氧化酶子单元1 (cox1) 基因设计了三对原料.
- 测序PCR产品,使用SeqMan软件将其拼接,并在MITOS2平台上注释开放的读取 (ORF).
- 使用Hd,FST,核酸多样性,GST,Tajima的D和Kimura-2参数遗传距离分析了遗传多样性.
主要成果:
- 获得了1536nt的全长cox 1基因序列,确认该物种是Hirudinaria manillensis.
- 与ITS (Hd=0.314,FST≈0) 相比,cox 1基因表现出较高的Hd (0.858) 和FST (0.10938) 值.
- 观察到低核酸多样性和GST值,非显著的负Tajima的D值,以及低遗传距离.
结论:
- 中国南部的Hirudinaria manillensis种群的数量增加,但缺乏稳定的遗传多样性和差异化.
- 这种模式归因于成熟的人工育种技术,有助于迁移和广泛繁殖.
- 这项研究强调了在公共数据库中需要更完整的Hirudinaria manillensis的cox 1和ITS基因序列数据.
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