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相关概念视频

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.5K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

932
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
932
Real Time RT-PCR02:57

Real Time RT-PCR

64.6K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.6K

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相关实验视频

Updated: Jan 15, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

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一个节奏基因表达的调度器.

Dimos Gaidatzis1,2, Maike Graf-Landua3,4, Stephen P Methot3

  • 1Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland. dimosthenis.gaidatzis@fmi.ch.

Molecular systems biology
|October 10, 2025
PubMed
概括
此摘要是机器生成的。

科学家们发现了基因振荡器如何使用模型生物精确地安排基因表达. 关键的转录因子对控制基因活动具有增强作用,为了解发育时间提供了一个框架.

关键词:
这里是C. elegans.染色是一种染色素.计算建模 计算建模发展时间表的发展时间表.振荡器的振荡器是什么

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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相关实验视频

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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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科学领域:

  • 发育生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 系统生物学 系统生物学

背景情况:

  • 遗传振荡器是发展和生理过程中精确定时基因表达的基础.
  • 了解控制大规模基因表达调度的调控机制对于破译生物复杂性至关重要.

研究的目的:

  • 为了研究基因振荡器如何调度成千上万个基因的表达,使用C. elegans变时钟作为模型.
  • 确定基因表达和染色体动态的基因表达和染色体动态的关键调节因素和机制.

主要方法:

  • 单细胞RNA测序以分析跨个体组织的基因表达模式.
  • 使用测序 (ATAC-seq) 来绘制染色体可访问性动态的转化酶可访问性染色体的时间解析测试.
  • 开发一款整合转录因子结合数据的线性模型,以预测色素动态.

主要成果:

  • 在个体组织中观察到广泛的峰值相分散,与数千个调节元件的节律色素可访问性变化相关.
  • 一个线性模型确定了九个关键的转录因子,其附加结合决定了调节元素的相位和振幅.
  • 证明这些因素也可以通过破坏性干扰来诱导构成性基因表达,正如GRH-1/Grainyhead扰动实验所证实的那样.

结论:

  • 建立了一个概念框架,以了解如何组合,非合作转录因子结合计划复杂的基因表达模式.
  • 这些发现提供了关于发育时间和其他动态生物过程的监管逻辑的见解.
  • 开发的模型准确地预测了转录因子扰乱对基因表达和染色质可访问性的影响.