微酸的电解水诱导金黄色葡萄球菌进入VBNC状态:浮游生物和生物膜状态之间的差异 细菌
Ziyi Zhang1, Jingqi Feng1, Haoran Cui1
1College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
Food research international (Ottawa, Ont.)
|October 11, 2025
概括
轻微酸性电解水 (SAEW) 有效地诱导了可活但不可培养 (VBNC) 状态在金黄色葡萄球菌中,这是一种通过食物传播的病原体. 这项研究详细介绍了SAEWEW.
科学领域:
- 食品微生物学 食品微生物学
- 抗菌剂 抗菌剂 抗菌剂
- 细菌生理学 细菌生理学
背景情况:
- 黄金葡萄球菌 (S. aureus) 是一种重要的食物传播病原体.
- 微酸电解水 (SAEW) 具有强大的抗微生物特性.
- 了解可生存但不可培养 (VBNC) 状态对于食品安全至关重要.
研究的目的:
- 为了评估SAEW对黄金菌VBNC形成的影响.
- 为了比较浮游生物与生物膜状态中的VBNC诱导.
- 阐明VBNC S. aureus. 的生理和遗传机制.
主要方法:
- 流细胞计分析VBNC状态形成.
- 测量ATP度,ROS度和呼吸情况.
- RT-qPCR用于评估基因表达变化.
主要成果:
- 在浮游生物和生物膜S. aureus中,SAEW诱导了VBNC状态.
- VBNC S. aureus 显示了代谢活动和基因表达的改变.
- 生物膜VBNC细菌的代谢水平较低,ROS比浮游生物更高.
结论:
- 在食品工业中,SAEW是一种有前途的S. aureus控制剂.
- 在浮游生物和生物膜VBNC状态下,SAEW会诱导不同的生理反应.
- 这项研究提供了对VBNC细菌控制策略的见解.
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