消化模式和通过不同过程产生的乳酸的生物活性释放:采用老年人动态胃肠道消化系统的体外研究
Mengqi Wang1, Xinyi Wu1, Wanyi Zhang1
1College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Food research international (Ottawa, Ont.)
|October 11, 2025
概括
在老年人中,冷干燥和喷雾干燥的乳酸消化揭示了结构分解和生物活性的释放. APVDAFK显示强烈的结合乙胆酶,表明潜在的治疗应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生化学
- 老年学是一门学科.
背景情况:
- 乳酸 (LF) 是一种具有各种健康益处的蛋白质.
- 了解老年人中LF消化对于优化其治疗效果至关重要.
- 不同的干燥方法,如冷干燥 (F-LF) 和喷雾干燥 (S-LF),可以影响LF结构和消化能力.
研究的目的:
- 在模拟老年人胃肠道疾病中描述F-LF和S-LF的体外消化行为.
- 为了识别在LF消化过程中释放的生物活性.
- 评估这些的与乙胆酶 (AChE) 相互作用的潜力.
主要方法:
- 使用一个体外动态消化系统 (DHSI-IV),模拟老年人的胃肠道状况.
- 分析了F-LF和S-LF的表面形态和蛋白质分解概况.
- 采用DIA相对定量组学来识别释放的.
- 进行了生物信息学分析和分子对接与ACHE.
主要成果:
- 喷雾干燥乳酸 (S-LF) 显示出比冷干燥乳酸 (F-LF) 更广泛的水解.
- F-LF和S-LF都耐胃水解,在60分钟后观察到显著的结构分解.
- 鉴定了许多,根据生物活性,毒性,可溶性和稳定性,选择了六种进行进一步分析.
- 酸APVDAFK通过键和疏水性相互作用与ACHE强烈结合,这是9.1.1的高-CDOCKER能量表明的.
结论:
- 在老年人胃肠道环境中,乳酸经历了显著的降解和结构变化.
- 消化LF释放生物活性,包括APVDAFK,这可能与关键酶如ACHE.交互.
- 这些发现为了解LF消化和老年人有益的生成提供了基础.
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