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麦子杂种之间的甲基组差异及其与基因组,转录组和表型变异的关联

Marius Kühl^1,2, Po-Ya Wu^1,2, Asis Shrestha^1,2

¹Institute of Quantitative Genetics and Genomics of Plants, Heinrich Heine University, Düsseldorf 40225, Germany.

Journal of experimental botany

|October 11, 2025

概括

麦子中的DNA甲基化影响复杂的特征和基因表达,独立于遗传序列变异. 这种表观遗传因素增强了基因组预测模型,为植物育种提供了新的见解.

科学领域:

植物基因组学植物基因组学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

表观遗传变异,特别是DNA甲基化,是理解复杂特征遗传性的关键.
研究春季大麦 (Hordeum vulgare亚种) 的全基因组甲基化. (vulgare) 对于提高作物质量至关重要.

研究的目的:

在大麦中表征全基因组甲基化模式.
探索甲基化,单核酸多态 (SNP) 和基因表达之间的关联.
评估DNA甲基化对基因组预测模型的影响.

主要方法:

全基因组双硫酸盐测序 (WGBS) 在23个亲属杂交线上.
差异甲基化区域 (DMR) 的识别和分析.
甲基化,SNP和基因表达之间的相关性分析,包括VRN-H1基因.

主要成果:

观察到高平均甲基化水平:88.6% (CpG),58.1% (CHG) 和1.4% (CHH).
确定了近50万个DMR,其中大多数缺乏序列变异.
在6%的DMR和基因表达之间发现了显著的关联,具有取决于位置的相关性模式.

结论:

基因甲基化在特征变异中起着独立的作用,与序列变异不同.
甲基化模式,如VRN-H1附近的甲基化模式,可以与表型特征 (如开花时间) 联系起来.
纳入DNA甲基化数据可以提高基因组预测模型对各种特征的准确性.

关键词:

大麦大麦,大麦大麦,大麦大麦,大麦大麦.通过DNA甲基化.生物信息学是一种生物信息学.基因调节基因调节基因调节自然变化的自然变化.植物表观遗传学

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