长非编码RNA Malat1和Neat1与牙周炎中的异生菌微生物组和上皮细胞-介质细胞过渡相关
Saif M Al-Mufti1,2, Ali A Abdulkareem1, Nibras H Chasib1
1College of Dentistry, University of Baghdad, Baghdad, Iraq.
Molecular oral microbiology
|October 11, 2025
概括
这项研究发现,长非编码RNAs NEAT1 和 MALAT1 与牙周炎中的上皮层-介质细胞过渡 (EMT) 有关. 这些分子可以作为牙周病的新型诊断生物标志物.
科学领域:
- 牙周医学 牙周医学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 表皮-介质细胞转换 (EMT) 在牙周炎的发病过程中起着作用,但其调节机制尚不清楚.
- 这项研究调查了长非编码RNAs (lncRNAs) NEAT1和MALAT1与牙周炎中EMT的关联.
研究的目的:
- 为了研究 lncRNAs NEAT1 和 MALAT1 与牙周炎中EMT的关联.
- 探索这些lncRNAs作为牙周炎的诊断生物标志物的潜力.
主要方法:
- 分析了来自57名牙周炎患者和健康对照组的牙组织样本.
- 使用RT-qPCR量化了EMT生物标记物 (E-cadherin,β-catenin,Snail1,vimentin) 和lncRNAs (NEAT1,MALAT1) 的表达水平.
- 组织病理学分析评估了炎症细胞透,并确定了细菌负载.
主要成果:
- 与对照组相比,牙周炎患者的临床参数和炎症细胞透率显著更高.
- 在牙周炎中,NEAT1,Snail1和维门丁的调节上升,而MALAT1和E-cadherin的调节下降.
- NEAT1,MALAT1,Snail1,E-cadherin和vimentin在区分牙周炎的诊断准确度中等至良好.
结论:
- NEAT1和MALAT1与牙周炎的EMT诱导有关.
- 临床参数,特定的细菌和NEAT1,MALAT1和E-cadherin的改变表达与EMT有关.
- 这些lncRNAs显示为牙周炎的新型诊断生物标志物具有前途.
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