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碳源介导的代谢和生物过程优化,用于Phormidium sp.中的C-phycocyanin丰富. 在双相静态混合营养培养下A02

Rajagopal Ramya¹, Seerappalli Aran Shanmugam², Ayyathurai Kathirvelpandian³

¹Institute of Fisheries Post Graduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Chennai, Tamil Nadu, 603 103, India. aymarpepsi@gmail.com.

Bioprocess and biosystems engineering

|October 11, 2025

概括

菌衍生C-Phycocyanin (C-PC) 产量被优化使用混合营养条件和特定的光波长. 红光诱导显著提高了C-PC含量,为合成色素提供了天然替代品.

科学领域:

生物技术和生物工艺工程
生理学和蓝藻细菌研究.
自然产品化学自然产品化学

背景情况:

色细菌中的光蛋白C-Phycocyanin (C-PC) 具有多种工业应用 (食品,化品,制药).
它的生物活性包括抗微生物,抗氧化,抗癌和抗炎性质.
福尔米迪亚 (Phormidium sp.) 是一种植物. A02是C-PC的潜在来源,为合成色素提供了替代品.

研究的目的:

优化生物工艺参数以提高生物质生产率和Phormidium sp.中的C-PC含量. A02.02 一个问题.
研究培养基,碳来源和光照条件对C-PC生产的影响.
开发一种可行的培养技术,用于工业规模的C-PC生产.

主要方法:

种植Phormidium sp. 的方法在混合变异的静态条件下A02.
补充各种碳来源 (葡萄糖,糖糖,) 和糖醇.
优化照明条件:光周期,强度和单色光 (红色,绿色,白色).

主要成果:

糖+子介质的C/N比率为4.76,与自身养对照相比,生物质生产率翻了一番.
对于C-PC增强的最佳甘油度是0.9g/L.
在双相培养策略中,红光诱导与白光相比,增加了6.5倍的C-PC含量.

结论:

使用优化的碳来源和光线策略进行混合养,可显著提高Phormidium sp.中的C-PC产量. A02.02 一个问题.
涉及红光诱导的两相静态培养策略有效地最大化了C-PC产量.
优化的Phormidium sp. 这种植物. A02种植是一种可行的生物工艺,用于生产C-PC作为天然色素.

关键词:

外源碳是一种外源碳.补充糖醇补充剂的使用方法绿色丝状蓝藻细菌是一种绿色丝状蓝藻细菌.代谢转换器的切换方式红灯感应红灯感应

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