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在对DNA损伤的反应中,AtGADD45A与支架蛋白AtRACK1A形成复合体

Xiaoyuan Peng¹, Ziyan Shi¹, Mengting Li¹

¹Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024, China.

Plant cell reports

|October 11, 2025

概括

植物应激传感器AtGADD45a1和AtGADD45a2与AtRACK1A结合,以调节Arabidopsis中的DNA损伤反应. 这种相互作用对于保持基因组完整性来应对环境压力因素至关重要.

科学领域:

分子生物学分子生物学
植物科学植物科学
遗传学遗传学是一个

背景情况:

DNA损伤威胁着植物基因组完整性,需要有效的DNA损伤反应 (DDR) 途径.
哺乳动物的DDR机制已得到充分研究,但植物的DDR仍未得到充分研究.
研究了哺乳动物生长停止和DNA损伤45A (GADD45A) 的Arabidopsis thaliana同类.

研究的目的:

描述阿拉比多普西斯GADD45A同类 (AtGADD45a1和AtGADD45a2) 在DNA损伤反应中的功能.
确定AtGADD45a1和AtGADD45a2.2的具有约束力的合作伙伴.
阐明AtGADD45a1/2-AtRACK1A复合体在植物DDR中的作用.

主要方法:

基因编辑CRISPR/Cas9基因编辑,以创建单个和双重突变.
用各种化学变异原体进行基因毒性测试.
免疫沉质谱测量以确定蛋白质相互作用.
预测AlphaFold3的结构.
用RT-qPCR分析基因表达.

主要成果:

单个突变 (atgadd45a1, atgadd45a2) 显示对基因毒素过敏,抑制了根的延长和减少了细胞的增殖.
双重突变是胚胎致命的,表明AtGADD45a1和AtGADD45a2.2之间的功能冗余.
确定了AtRACK1A作为AtGADD45a1/2.2.的一个有约束力的合作伙伴.
在AtGADD45a1/2-AtRACK1A复合体影响关键的DDR基因的表达.

结论:

在Arabidopsis中,AtGADD45a1和AtGADD45a2作为新的压力传感器.
这些蛋白与支架蛋白AtRACK1A相互作用,调节植物DNA损伤反应.
在保持植物基因组稳定性方面,AtGADD45a1/2-AtRACK1A复合体起着至关重要的作用.

关键词:

阿拉比多普西斯塔利亚纳.造成的DNA损伤是DNA损伤.增长停滞和DNA损伤-45-45 年.脚手架中的蛋白质

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