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相关概念视频

Repressible Operon: trp Operon01:21

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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相关实验视频

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超重叠的tRNA长非编码RNA位点抑制了代码偏差基因.

Sameen Ahmed1, Jacob L Fine2, Jordan J Chalmers1

  • 1Genetics and Genome Biology Program, SickKids Research Institute, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.

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概括

研究人员确定了tRNA重叠 lncRNAs (tROLs),这对软骨发育至关重要. 这些长非编码RNAs调节附近的基因和tRNA表达,作为非编码和编码基因组之间的桥梁.

关键词:
科普:分子生物学 分子生物学我们的 LINC0032424混凝土的生成 混凝土的生成基因调节 基因调节 基因调节基因组组织组织染色体间的接触是染色体之间的接触.在ncRNA中,我们可以这是一个tRNARNA.tROLOL 在线阅读

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.

背景情况:

  • 大多数长非编码RNAs (lncRNAs) 的功能在很大程度上是未知的.
  • 了解lncRNA的作用对于破译基因调节至关重要.
  • lncRNAs因其多样化的调节功能而越来越受认可.

研究的目的:

  • 描述一类新的 lncRNAs,称为tRNA重叠 lncRNAs (tROLs).
  • 研究tROLs在软骨发育和基因表达中的作用.
  • 阐明涉及tROLs,tRNA表达和蛋白质编码基因的调节机制.

主要方法:

  • 在体外软骨发育过程中调高的tROL基因的识别和特征.
  • 在tROL全基因删除后对基因表达变化的分析.
  • 调控下调的基因中的子偏差的研究.
  • 在tROL抑制或降解时评估tRNA表达水平.
  • 对tROL位置的染色体相互作用分析.

主要成果:

  • 在体外软骨发育过程中,tROL基因被上调.
  • tROL删除改变了编码器偏差基因的表达,特别是那些具有与重叠tRNA相对应的编码器的基因.
  • tROL位点位于基因密集区域,并表现出染色体间相互作用.
  • tROL全基因删除导致邻近基因的上调.
  • 对tROL转录的抑制或降解导致tRNA表达的减少.

结论:

  • tROL位置集体运作,依靠相互转录来调节附近的基因和调节tRNA表达.
  • tROLs作为非编码和编码基因组之间的监管联系.
  • 这项研究揭示了tROLs在基因调节和tRNA平衡中之前未被描述的作用.