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Updated: Jan 15, 2026

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具有蛋白质编码潜力的Bombyx mori核多重合体病毒衍生圆形RNA可促进病毒复制

Xing Zhang¹, Zeen Shen², Yani Kang¹

¹School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, China.

Insect biochemistry and molecular biology

|October 11, 2025

概括

这项研究表明,Bombyx mori核多重体病毒 (BmNPV) 产生增强病毒复制的病毒循环RNA (vcircRNAs). 一个vcircRNA转化了一种抑制宿主的.

科学领域:

病毒学病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

循环RNAs (circRNAs) 是稳定的RNA分子,在宿主病毒相互作用中发挥作用.
细菌病毒,如Bombyx mori核多重体病毒 (BmNPV),是昆虫的病原体.
了解病毒circRNA对于昆虫免疫研究至关重要.

研究的目的:

为了研究 circRNAs 在 Bombyx mori. 的 BmNPV 感染期间的作用.
为了识别和描述由BmNPV.产生的病毒circRNAs (vcircRNAs).
阐明vcircRNAs在病毒复制和宿主免疫逃避中的功能.

主要方法:

高通量测序以识别宿主和病毒circRNAs.
实验验证 circRNA 的存在.
在体外翻译测试以确定从vcircRNAs中产生的蛋白质.
功能性测试用于评估vcircRNA及其编码对病毒复制的影响.
机制研究侧重于RNA干扰 (RNAi) 途径.

主要成果:

在BmNPV感染期间,发现了数百个宿主circRNA和众多病毒circRNA (vcircRNA).
BmNPV编码为vcircRNA-390,它包含一个开放的读取框架 (ORF) 和内部核糖体进入点 (IRES).
vcircRNA-390被翻译成一个81-氨基酸病毒 (VSP81),增强病毒复制.
VSP81针对宿主RNA干扰 (RNAi) 途径,促进病毒免疫逃避.

结论:

在BmNPV感染中,vcircRNA-390是一种新型的前病毒因素.
BmNPV利用circRNA介导的翻译来增强病毒复制并逃避宿主抗病毒防御.
这些发现扩大了对病毒circRNA生物学和宿主-病原体相互作用的理解.

关键词:

在BmNPVV中.在VSP81中使用VSP81.病毒的 circRNA 病毒的 circRNA 病毒复制是病毒的复制.这就是vcircRNA-390的原因.

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