B型HBV表面抗原抗原抗原循环的对称结构
Weiyu Tao1, Xiao He1, Lei Chen2
1State Key Laboratory of Membrane Biology, College of Future Technology, Institute of Molecular Medicine, Peking. University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China.
Journal of molecular biology
|October 11, 2025
概括
乙型乙型肝炎病毒 (HBV) 抗原循环 (AGL) 结构显示了双重对称性,使两种中和抗体片段能够同时结合. 这一发现澄清了HBV免疫逃避机制.
科学领域:
- 结构生物学 结构生物学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 乙型肝炎病毒 (HBV) 表面抗原 (HBsAg) 是唯一的病毒膜蛋白.
- HBsAg的细胞外抗原循环 (AGL) 对病毒附着,抗体向至关重要,并且容易逃脱突变.
- 之前的研究表明了两种AGL结构 (A型和B型),但缺乏B型的完整模型和对称细节.
研究的目的:
- 为了确定AGL型B的完整的冷电子显微镜 (cryo-EM) 结构.
- 阐明AGLB型的对称性和结合特性.
- 了解特定中和抗体 (NAb) 全血型活性和逃脱突变抵抗背后的机制.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 确定了AGL型B的结构.
- 该结构与中和抗体H020.的Fab片段进行了复合分析.
- 结构分析的重点是对称性,结合点,以及对抗体相互作用的影响.
主要成果:
- 解决了AGL Type B的完整的冷EM结构,揭示了它的双重对称性.
- 证明AGL Type B可以同时结合NAb H020的两个Fab片段.
- 结构数据提供了关于NAb H020泛血型中和机制的见解,以及逃生突变如何影响结合.
结论:
- 解决了AGL Type B的结构,使其完整的形状和对称性变得清晰.
- 两个抗体片段的同时结合解释了NAb H020的广泛中和能力.
- 了解这些相互作用是制定抗HBV免疫逃逸策略的关键.
更多相关视频
08:09Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
10.0K
10:50Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
Published on: September 15, 2010
9.9K
相关概念视频
01:10Antibody Structure
65.3K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.3K
01:25Antibody Structure and Classes
8.2K
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
8.2K
01:24B Cell Activation and Differentiation
16.0K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
16.0K
01:28Diversity of Antigen Receptors
1.4K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.4K
01:26Antigens Involved in Adaptive Immunity
1.3K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
1.3K
02:28Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K