通过DEPR1-SERK2受体综合体识别植物细胞因子,使小麦具有多种病原体的耐药性
Lijun Wang1, Jun Zhao2, Chuanchun Yin2
1State Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Weifang, China; College of Plant Protection, Shandong Agricultural University, Tai'an, China.
研究人员在小麦中发现了一种新的植物免疫信号通路,涉及三角洲类植物细胞因子 (DEP). 这一途径增强了对多种病原体的耐药性,为作物保护提供了新的策略.
科学领域:
- 植物免疫学 植物免疫学
- 分子植物病理学 分子植物病理学
- 小麦遗传学 小麦遗传学
背景情况:
- 植物性免疫性 (植物细胞因子,PCK) 激活了植物的免疫反应.
- 小麦中PCK介导免疫的分子机制在很大程度上是未知的.
研究的目的:
- 识别和表征一种参与植物免疫的新型小麦PCK.
- 阐明小麦PCK感知和信号传导的分子机制.
- 评估这种途径在小麦中设计多种病原体耐药性的潜力.
主要方法:
- 一个小麦PCK的识别和功能分析,类似于delta的PCK (DEP).
- 通过富含白的重复受体激酶 (LRR RKs) DEPR1和SERK2.2研究DEP感知.
- 低温电子显微镜用于对DEP-DEPR1-SERK2复合物的结构分析.
- 测试以确定小麦对Xanthomonas透光和Fusarium物种的耐药性.
主要成果:
- 一种新的小麦PCK,三角形类PCK (DEP) 被确定并被证明可以激活免疫反应.
- DEP被DEPR1和SERK2感知,从而触发了依赖DEPR1和SERK2的信号通路.
- 结构分析显示,DEP招募了SERK2到DEPR1,促进了异构化.
- 该DEP-DEPR1-SERK2模块赋予了对Xanthomonas透光,Fusarium graminearum和Fusarium pseudograminearum的耐药性的作用.
- 该模块通过对抗酸信号来增强耐药性.
结论:
- 在小麦中发现了一种新的植物细胞因子介导的免疫信号通路,涉及DEP,DEPR1和SERK2.
- 这一途径提供了对主要小麦病原体的广泛耐药性.
- 这些发现为开发耐多病原体的小麦品种提供了有前途的战略.
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