相关实验视频

Updated: Jan 15, 2026

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

一个新的UDP-glycosyltransferase用于来自Gynostemma pentaphyllum (Thunb.) 的罕见人参酸生物合成

Qiaoxiang Wu¹, Yangyang Chen¹, Mingxing Ye¹

¹Institute of Structural Pharmacology & TCM Chemical Biology, College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China.

Carbohydrate research

|October 12, 2025

概括

研究人员发现了一种新的酶,GpUGT74A1,来自Gynostemma pentaphyllum. 这种酶有助于产生有价值的金氏化物,扩大了罕见金氏化物生物合成的选择.

科学领域:

生物技术是生物技术.
植物生物化学植物生物化学
酶工程是什么?酶工程是什么?

背景情况:

银酸具有显著的治疗潜力,推动了对其生产的研究.
UDP-glycosyltransferases (UGTs) 是人参酸生物合成的关键酶,但有限的可用性阻碍了生产品种和产量.
昆科植物Gynostemma pentaphyllum是与人参化物相关的酶的独特来源.

研究的目的:

为了识别和描述新型的UDP-glycosyltransferases (UGTs) 来自Gynostemma pentaphyllum用于生化合成的生化合成.
探索GpUGT74A1在罕见人参化物异质合成中的潜力.
克服生物技术应用的酶表达和活性方面的挑战.

主要方法:

来自G. pentaphyllum的新型葡萄糖转移酶的采矿和序列分析,该新型葡萄糖转移酶被指定为GpUGT74A1.1.
使用MBP融合标签在大肠杆菌中对GpUGT74A1的可溶性表达.
在体外酶活性测试以确定GpUGT74A1在特定的金氏化物前体上的糖化能力.

主要成果:

在G. pentaphyllum中发现了一种属于UGT74家族的新型UGT,GpUGT74A1.
使用MBP标签实现了GpUGT74A1的可溶性表达,克服了E. coli中的不可溶性问题.
GpUGT74A1显示了对PPD,PPT和Rh2的C-20糖化酶的催化活性,分别产生CK,F1和F2.

结论:

发现GpUGT74A1的发现丰富了植物衍生型葡萄糖转移酶基因的谱.
GpUGT74A1代表了一种有前途的新候选酶,用于高效的罕见和有价值的雌激素酶的异质合成.
这项研究为工程UGT提供了基础,以增强用于制药应用的各种人参化物的生产.

关键词:

生物合成生物合成生化物是什么?吉诺斯泰玛五植物 (Gynostemma pentaphyllum) 是一种植物.在UDP-glycosyltransferase中使用.

更多相关视频

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

相关概念视频

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...