空间时空多元组学 在芯片上对活单细胞进行剖析,用于癌症治疗
Kunru Yu1, Shengjie Chen1, Rong Zhu1
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China.
ACS sensors
|October 13, 2025
概括
这项研究使用微芯片实时实地对单个癌细胞进行分子监测. 这项技术揭示了瘤治疗场 (TTFields) 如何影响癌细胞通路,帮助精确治疗.
科学领域:
- 生物技术是生物技术.
- 癌症研究 癌症研究
- 分子生物学分子生物学
背景情况:
- 单细胞分子分析对于理解细胞异质性和生物过程至关重要.
- 现有的细胞监测方法往往是终点测量,限制了对活细胞的长期研究,特别是在癌症治疗中.
- 癌症中瘤治疗场 (TTFields) 的精确分子机制在很大程度上是未知的.
研究的目的:
- 开发一种多功能微芯片,用于在现场持续监测活单细胞中的分子特征.
- 在癌症治疗中研究TTFields的分子机制,使用时空多态学分析.
- 为了实时跟踪TTFields治疗下癌细胞中的分子事件.
主要方法:
- 在单个微芯片上整合空间细胞定位,用于多分子跟踪的表面增强拉曼散射 (SERS) 和TTFields处理.
- 用TTFields和化疗药物治疗的肝癌细胞的多重分析.
- 空间-时间多态学分析,包括对糖解,遗传稳定性和膜完整性的跟踪.
主要成果:
- 微芯片平台成功实现了在现场持续监测活单细胞中的分子动力学.
- 在TTFields治疗和未治疗的癌细胞之间观察到分子事件 (糖解,遗传稳定性,膜完整性) 的显著差异.
- 因果网络分析确定了由TTFields调节的特定瘤性途径.
结论:
- 开发的微芯片在癌症治疗期间对活细胞中的分子变化进行实时监测是有效的.
- 这个平台提供了对TTFields分子机制的见解,支持精确癌症治疗的开发.
- 该研究强调了集成微流体设备在促进癌症研究和治疗策略方面的潜力.
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