在MYB转录因子基因BjPur中插入内源转位子,通过诱导Brassica juncea中的表观遗传修饰来扰乱安托西亚宁积累
Qiqi Cheng1, Sihan Dong1, Liping Guo1
1College of Horticulture and Forestry Sciences, Huazhong Agricultural University, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Wuhan, 430070, China.
The New phytologist
|October 13, 2025
概括
像Bjpur-SINE逆转移子这样的可转移元素 (TEs) 可以使Brassica juncea的宿主基因沉默. DNA和基因素甲基化是控制这种表观遗传沉默的关键机制,影响叶子颜色.
科学领域:
- 植物遗传学 植物遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 可转移的元素 (TE) 包含超过50%的Brassica juncea基因组.
- 对宿主基因表达和基因组进化的TEs的影响是显著的,但尚未完全理解.
- 以前的研究发现了B. juncea.中影响叶子颜色的DNA插入.
研究的目的:
- 确定导致B. juncea.叶子颜色变化的DNA插入的性质.
- 为了阐明由逆转移素介导的基因沉默的分子机制.
- 调查DNA和基因素甲基化在调节宿主基因表达中的作用.
主要方法:
- 一个短间隔的核元素 (SINE) 逆转移子的识别,Bjpur-SINE.
- 在BjPur位点对DNA甲基化模式的分析.
- 用于DNA甲基化抑制剂的应用.
- 针对DNA甲基化因子的基因淘汰实验 (BjDRM2,BjAGO4).
- 评估基因素修饰的情况.
主要成果:
- 叶子改变颜色的DNA插入被确定为Bjpur-SINE逆转移子.
- 插入Bjpur-SINE导致BjPur基因的DNA甲基化和转录抑制增加.
- DNA甲基化抑制剂和BjDRM2/BjAGO4的淘汰作用逆转了沉默效应.
- 在绿叶植物的BjPur位点观察到抑制性组织蛋白修饰.
结论:
- 在B. juncea.中,DNA和基因素甲基化协同介导逆转移素诱导的表观遗传沉默.
- 内部可移植元素可以通过表观遗传机制调节宿主基因表达.
- 这项研究提供了对TE-宿主基因相互作用及其对植物表型的影响的见解.
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