骨质母细胞和骨质母细胞中的旁观者效应:X辐射和质子辐射之间的比较
Noriyuki Okonogi1,2,3, Yukako S Otani1, Keisuke Otani1
1Department of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
International journal of particle therapy
|October 13, 2025
概括
辐射诱导旁观者反应 (RIBR) 发生在骨细胞中,如骨质细胞和骨质细胞. 这些效应在X射线和质子中都被观察到,在辐射类型或剂量之间没有显著差异.
科学领域:
- 辐射生物学 辐射生物学
- 细胞生物学 细胞生物学
- 骨生物学 骨生物学
背景情况:
- 辐射诱导旁观者反应 (RIBR) 是细胞间信号事件,非辐射细胞在暴露于辐射后表现出遗传不稳定性.
- 了解RIBR对于精确评估放射治疗和职业暴露的风险至关重要,特别是在与骨相关的组织中.
研究的目的:
- 研究骨质细胞和骨质细胞中辐射诱导的旁观者反应 (RIBR).
- 为了比较这些骨细胞中X射线和质子诱导的RIBR效应.
主要方法:
- 使用的MC3T3-E1 (前骨质细胞/骨质细胞) 和RAW264.7 (骨质细胞) 细胞系.
- 采用跨井插入培系统来研究中介RIBR.
- 用X射线和质子 (2.3keV/μm) 照射细胞,剂量范围为0.5至2 Gy.
- 通过测量旁观细胞中的53BP1表达和微核形成来评估RIBR.
主要成果:
- 在旁观者细胞中观察到RIBR,无论细胞组合 (骨质细胞-骨质细胞,骨质细胞-骨质细胞或混合).
- 与对照细胞相比,在旁观细胞中发现了53BP1阳性细胞和微核形成的显著增加 (P < .05).
- 在X射线和质子辐射之间没有发现RIBR的统计学上显著差异,在测试范围内也没有剂量依赖的影响.
结论:
- 初步证据表明,RIBR发生在骨质母细胞和骨质母细胞中.
- 在与骨相关的细胞中,X射线和质子似乎都会诱导类似的旁观者效应.
- 这些发现表明,辐射类型和剂量可能不会在这些细胞类型中显著改变RIBR.
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