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通过电气发酵解锁1,3-二醇的产生,由 Pseudomonas aeruginosa 通过电气发酵

Julia Pereira Narcizo¹, María-Eugenia Guazzaroni², Adalgisa Rodrigues de Andrade¹

¹Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Ribeirão Preto, SP 14040-901, Brazil.

|October 13, 2025

概括

使用Pseudomonas aeruginosa的电发酵显著提高了从甘油中产生的1,3-propanediol (1,3-PDO) 的产量. 这种生物电化学方法提高了产量,并提供了一种可持续的方法来生产这种有价值的化学构件.

科学领域:

生物技术是生物技术.
生物化学工程生物化学工程
微生物电化学微生物电化学

背景情况:

1,3-二醇 (1,3-PDO) 是一个重要的工业化学品和聚合物前体.
作为生物柴油副产品的甘油是1,3-PDO生产的可持续原料.
传统的1,3-PDO发酵方法在产量和效率方面存在局限性.

研究的目的:

为了研究使用Pseudomonas aeruginosa进行1,3-propanediol (1,3-PDO) 生产的电发酵的有效性.
通过电发酵与传统发酵进行1,3-PDO生产的比较.
探索应用电压对1,3-PDO生物合成和代谢途径的影响.

主要方法:

使用一个单微生物电化学电池,配有碳布和电极.
在不同应用电压 (0.010.4V) 和潜在静电控制下进行电发酵试验.
使用Pseudomonas aeruginosa用于将甘油生物转化为1,3-PDO.
分析了1,3-PDO度,产量,生产率和电子回收.

主要成果:

与传统发酵相比,电气发酵显著提高了1,3-PDO生产.
0.05V的最佳电压导致1,3-PDO度为99.49 ± 0.57 mmol L-1,产量为0.78 ± 0.01 mol/mol,生产率为15.70 ± 0.25 mmol L-1 h-1.
与传统发酵 (45.14 ± 0.89%) 相比,电子回收在电发酵 (89.65 ± 0.37%) 中明显高,表明更有效的还原途径.

结论:

电气发酵有效调节Pseudomonas aeruginosa的新陈代谢,以提高1,3-PDO的生产.
这种生物电化学方法为从糖中生产1,3-PDO提供了一个有希望的,可持续的替代方案.
这些发现支持将电发酵集成到生物炼油厂中,用于增值化学合成.

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