转化合成DNA聚合酶基因表达受年龄和IGF-1在表皮人体皮肤中的影响
Stanley D Rider1, Madison S Owens1, Craig A Rohan1,2,3
1Department of Pharmacology & Toxicology, Wright State University Boonshoft School of Medicine, Dayton, Ohio, USA.
JID innovations : skin science from molecules to population health
|October 13, 2025
概括
老年人的皮肤在UVB暴露后显示转化合成 (TLS) DNA聚合酶基因表达的改变,这可能会影响老年人的皮肤癌症风险. 这表明需要考虑老年皮肤癌研究中的TLS变化.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 老年人暴露在阳光下的皮肤容易患癌症,原因是DNA修复受损.
- 转化合成 (TLS) DNA聚合酶对于复制受损DNA至关重要,特别是在紫外线暴露后.
研究的目的:
- 为了研究TLS DNA聚合酶在年轻人与老年人的皮肤中的表达.
- 为了确定老年皮肤是否更多地依赖TLS聚合酶,用于未经修复的紫外线光产品.
- 探索UVB暴露对不同年龄组TLS聚合酶表达的影响.
主要方法:
- 在年轻和老年人的皮肤表皮中检查了TLS聚合酶基因表达 (POLH,POLI,POLK,REV1,REV7).
- 与皮肤衰老生物标志物相关的TLS基因表达 (COL1A1,S100A7).
- 评估了UVB辐射对TLS聚合酶mRNA水平在培养的角质细胞和皮肤样本中的影响.
主要成果:
- 在老年皮肤中,TLS聚合酶基因表达与COL1A1相反相关.
- 与年轻皮肤相比,紫外线暴露导致老年皮肤的POLI,POLK和REV1mRNA水平升高.
- 缺乏胰岛素样生长因子-1信号增强UVB诱导的REV1表达.
结论:
- 老年人的皮肤在UVB照射后表现出改变的TLS聚合酶基因表达.
- 这些变化可能在老年人对皮肤致癌的敏感性增加中起作用.
- 未来人类皮肤癌研究应该考虑老年皮肤TLS聚合酶基因表达的变化.
更多相关视频
10:25Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron dgn-destabilized Green Fluorescent Protein GFP-based Reporter Protein
Published on: November 10, 2012
17.2K
08:45Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
Published on: March 18, 2016
7.2K
相关概念视频
01:12Renewal of Skin Epidermal Stem Cells
3.0K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
3.0K
02:10Translesion DNA Polymerases
11.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.0K
01:38Nucleotide Excision Repair
5.0K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.0K
01:08Nucleotide Excision Repair
40.6K
Overview
40.6K
01:29Replication in Eukaryotes
17.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
17.1K
01:19The Effect of Aging on Tissues
3.3K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.3K