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相关概念视频

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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在活细胞中以图像为基础进行分析,使用活细胞绘画.

Thaís Moraes-Lacerda1, Mariana Rodrigues-Da-Silva1, Shantanu Singh2

  • 1Departamento de Bioquímica e Biologia Tecidual, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil.

Bio-protocol
|October 13, 2025
PubMed
概括

本研究引入了一种具有成本效益的活细胞高含量分析 (HCA) 方法,使用酸. 这种方法可以通过实时可视化亚细胞结构来实现动态细胞分析和药物发现.

科学领域:

  • 细胞成像和分析.
  • 药物发现和毒理学
  • 现象类型概况分析

背景情况:

  • 高含量分析 (HCA) 传统上使用固定细胞,限制动态过程观察.
  • 活细胞成像为研究实时细胞反应提供了扩展的能力.
  • 现有的固定细胞测试在检测微妙,次致命的表型变化方面存在局限性.

研究的目的:

  • 提出一个简单,成本效益高,可扩展的活细胞HCA协议.
  • 为了实现实时的表型分析和剂量反应分析.
  • 通过保持细胞活力和使动态测量成为固定细胞测试的补充.

主要方法:

  • 使用阿克里丁色 (AO),一种元色光染料,用于活细胞HCA.
  • 采用双通道光读数可视化核和酸性隔间.
  • 整合了高通量显微镜和计算分析的方法.

主要成果:

  • 在活细胞中展示了明显的亚细胞结构的可视化.
  • 启用了各种扰动物的表型分析和剂量反应分析.
  • 为检测微妙,次致命的表型变化提供了一个框架.

结论:

关键词:
阿克里丁色染色剂的色染色光显微镜的光学显微镜.高含量选 高含量的选基于图像的表型鉴定.活细胞成像成像技术

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  • 基于AO的活细胞HCA协议提供了一个多功能平台,用于发现复杂的细胞表型.
  • 这种方法补充了固定细胞检测,增强了药物发现和毒理学研究.
  • 精简的分析管道确保了基于图像的数据的高效和可重现的解释.