在Baijiuu中对病毒动态和功能进行metagenomic洞察
Huadong Zhang1, Hongxia Zhang2, Hai Du1
1Laboratory of Brewing Microbiology and Applied Enzymology, The Key Laboratory of Industrial Biotechnology, Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, 1800 Lihu Ave, Wuxi, 214122, Jiangsu, China.
Current research in food science
|October 13, 2025
概括
这项研究揭示了Baijiu发酵中的病毒动态,确定了关键的病毒家族及其宿主. 病毒社区的变化与微生物继承和环境因素有关,突出显示了病毒在这个复杂生态系统中的作用.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- 白发酵涉及复杂的微生物联盟,但病毒的作用尚不清楚.
- 现有的研究主要集中在细菌和真菌对发酵的贡献上.
研究的目的:
- 在 Baijiu 发酵过程中,利用元基因组学分析病毒社区动态.
- 为了确定关键的病毒家族,宿主,以及它们与环境因素和代谢物的关系.
主要方法:
- 在五个不同的发酵阶段 (0-30天) 进行发酵谷物的元基因组测序.
- 生物信息分析用于识别病毒家族,预测菌体与宿主相互作用,并分析病毒辅助代谢基因 (AMG).
- 多变量分析以将病毒动态与微生物继承,环境参数和代谢物相关联.
主要成果:
- 确定了101个病毒家族,其中Metaviridae,Parvoviridae和Aliceevansviridae占主导地位.
- 乳酸细菌被确定为主要的菌体宿主.
- 病毒群体的组成从ssRNA和ssDNA病毒 (早期阶段) 转移到dsDNA病毒 (后期阶段).
- 病毒动力学受到微生物继承,温度,pH值,水分,葡萄糖和乳酸,酸和乙醇等代谢物的显著影响.
- 参与氨基酸代谢和维生素生物合成的病毒AMG在第30天显著增加.
结论:
- 这项研究为Baijiu发酵生态系统中的病毒生态提供了新的见解.
- 病毒群体在Baijiu发酵的复杂生化过程中发挥着重要作用.
- 了解这些病毒与宿主相互作用对于优化发酵过程至关重要.
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