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在前列腺癌中交叉来源的miRNA生物标记物的系统性表征,使用计算-实验综合分析

Huimin Lu¹, Wenjin Li², Zhongxin Huang³

¹Department of Urology and Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Frontiers in cell and developmental biology

|October 13, 2025

概括

这项研究确定了关键的microRNAs (miRNAs) 并构建了前列腺癌 (PCa) 的miRNA-mRNA网络. 血清和组织中的下调 miR-136-3p 表明其作为PCa生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

前列腺癌 (PCa) 往往是晚期诊断的,一旦转移,很难治疗.
识别像microRNAs (miRNAs) 这样的关键分子参与者对于理解PCa进展至关重要.
开发新的诊断和治疗策略需要对miRNA-mRNA相互作用的深入知识.

研究的目的:

为了确定涉及前列腺癌的关键miRNAs.
构建针对PCa.特定的调节miRNA-mRNA网络.
发现PCa.潜在的新生物标志物和治疗点.

主要方法:

对大型miRNA表达数据集的分析 (GSE112264,TCGA).
使用miRTarBase和途径分析 (GO,KEGG) 预测miRNA目标基因.
构建蛋白质-蛋白质相互作用网络 (STRING,Cytoscape) 并使用UALCAN数据库进行验证.

主要成果:

两种重叠下调的miRNAs (miR-146a-3p和miR-136-3p) 被确定,针对948个基因.
丰富分析将DE-miRNA与PCa相关途径联系起来,包括mTOR信号传递.
四个枢纽基因 (NSF,HIST2H2BE,IGF2R,CADM1) 被确定并显示异常表达;miR-136-3p在血清和组织中显著降低.

结论:

成功建立了一个针对PCa的全面miRNA-mRNA网络.
miR-136-3p显示出作为诊断生物标记物的潜力,因为其在PCa血清和组织中的表达减少.
已确定的网络和枢纽基因为未来的PCa研究和治疗开发提供了有前途的途径.

关键词:

生物信息学是一种生物信息学.生物标志物生物标志物这是一个微型RNA.前列腺癌是前列腺癌.监管网络监管网络监管网络监管网络监管

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...