Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.4K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

B cells enable autoreactive T cells to avoid suppression.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multiple Mechanisms Contribute to Robust Type 1 Diabetes Protection in Nfkbid- Overexpressing NOD Mice.

Diabetes·2026
Same author

Direct-in-NOD genetic ablation of Bcl3 leads to complete type 1 diabetes protection.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Biological rhythm patterns and internalising disorders among children and adolescents: a cross-sectional study.

BMJ open·2025
Same author

Advancing Animal Models of Human Type 1 Diabetes.

Cold Spring Harbor perspectives in medicine·2024
Same author

Murine MHC-Deficient Nonobese Diabetic Mice Carrying Human HLA-DQ8 Develop Severe Myocarditis and Myositis in Response to Anti-PD-1 Immune Checkpoint Inhibitor Cancer Therapy.

Journal of immunology (Baltimore, Md. : 1950)·2024
Same journal

Decoding Viral Dark Matter: Metagenomic Prokaryotic Virus Characterization With Pharokka, Phold, and Phynteny.

Current protocols·2026
Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026
Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026
Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026
Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026
Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026
查看所有相关文章

相关实验视频

Updated: Jan 15, 2026

Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model
06:22

Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model

Published on: November 29, 2024

1.3K

针对1型糖尿病的人性化小鼠模型.

David V Serreze1, Marissa Tousey-Pfarrer1, Jeremy J Racine1

  • 1The Jackson Laboratory, Bar Harbor, Maine.

Current protocols
|October 13, 2025
PubMed
概括
此摘要是机器生成的。

使用CRISPR/Cas9将NOD小鼠与患者特定的HLA和T细胞受体人性化,为1型糖尿病 (T1D) 研究创造了更好的模型. 这种方法促进了有效的T1D干预措施的发展.

关键词:
在MHC/HLA中.对于TCRs来说,这是一个很好的方法.自免疫型T1D.人性化的小鼠.

更多相关视频

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

10.0K
Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
10:03

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Published on: November 18, 2022

2.2K

相关实验视频

Last Updated: Jan 15, 2026

Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model
06:22

Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model

Published on: November 29, 2024

1.3K
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

10.0K
Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
10:03

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Published on: November 18, 2022

2.2K

科学领域:

  • 免疫学 免疫学 免疫学
  • 遗传学 遗传学 是一个
  • 内分泌学 在内分泌学.

背景情况:

  • 1型糖尿病 (T1D) 是一种自身免疫性疾病,主要由T细胞驱动,受多基因因素的影响.
  • 主体组织相容性复合体 (MHC) 和人类白细胞抗原 (HLA) 变体是T1D的关键遗传风险因素.
  • 目前的NOD小鼠模型提供了洞察力,但在将发现转化为人类T1D时存在局限性.

研究的目的:

  • 审查T1D研究中人性化的NOD小鼠模型的开发和应用.
  • 探索使用CRISPR/Cas9技术来创建这些先进的临床前模型.
  • 突出这些模型在确定临床相关的T1D干预策略方面的潜力.

主要方法:

  • 使用CRISPR/Cas9基因编辑来生成缺少小鼠MHC的NOD小鼠.
  • 将人类的HLA和T细胞受体 (TCR) 分子引入这些改性小鼠.
  • 描述了由此产生的人性化小鼠模型,用于它们的T1D病变发生.

主要成果:

  • 克里斯普尔/Cas9能够创造出缺乏MHC的小鼠NOD小鼠.
  • 这些小鼠可以被设计成表达与患者相关的HLA和TCR分子.
  • 人性化的模型更好地回顾了人类T1D的发展,为研究提供了改进的平台.

结论:

  • 人性化的NOD小鼠代表了临床前T1D研究的重大进展.
  • 这些模型有助于开发和测试新的,临床上可转化T1D干预措施.
  • 这种方法对推进T1D和其他自身免疫性疾病的治疗有前途.