使用无动物成分介质优化和可扩展生产Corynebacterium diphtheriae毒素:通往更安全的疫苗开发的途径
Prashant R Chawla1, Uma Addepally2
1Center of Biotechnology, University College of Engineering, Science and Technology, JNTUH, Kukatpally, Hyderabad, 500085, India.
Archives of microbiology
|October 13, 2025
概括
这项研究优化了使用无动物介质的喉毒素 (DT) 生产,通过改进铁度和工艺参数来实现更高的产量. 在生物反应器中证实了可扩展性,证明了高效的DT制造潜力.
科学领域:
- 生物技术是生物技术.
- 生物工艺工程 生物工艺工程
- 疫苗开发 疫苗开发
背景情况:
- 脊髓灰质炎仍然是一个重要的传染病,需要有效的疫苗生产.
- 传统的喉毒素生产依赖于来自动物的介质,这带来了一些局限性.
- 优化喉毒素 (DT) 生产对于疫苗供应至关重要.
研究的目的:
- 为了增强喉毒素 (DT) 产量,使用无动物成分的半合成介质.
- 优化铁度和关键工艺参数,以获得最大的DT产量.
- 为了验证优化 DT 生产过程的可扩展性.
主要方法:
- 在半合成介质中研究的铁度 (0.050.6μg/mL).
- 采用响应表面方法来优化pH值,温度,振动和注射度.
- 在5升水箱生物反应器中验证了该过程,评估了通风和振动效应.
主要成果:
- 确定了0.4μg/mL的最佳铁度,产生了221 Lf/mL的DT.
- 优化的条件 (pH 7.2,35°C,150rpm,5.8%的注射剂) 导致245 Lf/mL DT.
- 在600rpm和0.25VVM的生物反应器验证实现了266Lf/mL DT,而更高的空气化/化对产量产生了负面影响.
结论:
- 优化的工艺参数和铁度显著提高了在无动物介质中的DT生产.
- 开发的可扩展战略证明了有效和改进的喉毒素制造.
- 这种方法提供了一个可行的替代方案,以传统的方法为喉毒素生产.
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