在患有牙周炎的MAFLD患者中分析下微生物群落结构
Hai He1, Jingli Zhu2, Chuyi Li3
1Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, No. 1 Xibeixincun Baiyin Road, Chengguan District, Lanzhou, Gansu Province, 730030, China.
Clinical oral investigations
|October 13, 2025
概括
与牙周炎 (PD) 相关的代谢性脂肪肝病 (MAFLD) 显示出明显的下微生物群变化,包括增加的促炎细菌. 这些发现表明,MAFLD通过改变口腔微生物群落来加剧牙周炎.
科学领域:
- 口腔微生物学 口腔微生物学
- 胃肠病学 胃肠病学
- 牙周病学 牙周病学
背景情况:
- 与代谢相关的脂肪肝疾病 (MAFLD) 和牙周炎 (PD) 是普遍存在的慢性疾病.
- 人们越来越认识到系统性疾病和口腔微生物群之间的相互作用.
- 下微生物群的改变与各种口腔和全身疾病有关.
研究的目的:
- 研究患有PD的MAFLD患者中下微生物群的病理关联.
- 为了比较MAFLD-PD患者和PD患者之间的下菌群组成,多样性和功能.
- 分析细菌分类,代谢途径和群体之间的表型之间的差异.
主要方法:
- 来自15名MAFLD-PD患者和15名PD患者的下斑块样本的16S rRNA基因测序.
- 使用Alpha和Beta多样性指标分析微生物社区结构.
- LEfSe用于物种歧视,PICRUSt2用于功能预测,BugBase用于表型预测.
主要成果:
- 在MAFLD-PD和PD组之间,Alpha多样性 (Chao1,Shannon,Simpson) 和Beta多样性 (PCoA) 的显著差异.
- 在MAFLD-PD患者中增加了蛋白质细菌和活性细菌的相对丰富性,并减少了Bacteroidota,Porphyromonas和Treponema.
- 在KEGG路径的潜在差异趋势和群体之间的4个表型的显著差异.
结论:
- 在MAFLD-PD患者的下斑块表现出促炎细菌,代谢途径障碍和增强的致病性.
- MAFLD可能会通过改变下微生物群落结构来恶化PD的进展.
- 提供了针对微生物群的治疗方法和MAFLD-PD的多学科干预措施的理论基础.
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