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Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
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由Bacillus分离物中基托酶产生的基托寡糖的免疫调节作用
Akram N Salah1, Nooran S Elleboudy2, Mohamed M S Farag3,4,5
1Experimental and Advanced Pharmaceutical Research Unit, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Abbassia, Cairo, 11566, Egypt.
AMB Express
|October 13, 2025
概括
从Bacillus cereus中生物制造的奇托寡糖 (COSs) 在小鼠中显示出显著的免疫刺激和抗炎作用. 这些发现突出了COSs.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 素寡糖化物 (COSs) 是水溶性,具有已知的生物活性的生物可用寡聚物.
- 以前的研究表明,COS具有抗瘤,抗微生物,抗氧化和免疫调节的特性.
- 对新型免疫刺激和抗炎药物的需求推动了对生物产生的COS的研究.
研究的目的:
- 使用本地Bacillus cereus菌株生物制造和净化奇托寡糖 (COSs).
- 研究纯化COSs对免疫抑制小鼠的免疫刺激作用.
- 为了评估纯化COSs的抗炎潜力,在卡拉根诱导的胀模型中.
主要方法:
- 生物生产的COSs通过发酵的B. cereus与合物奇托桑.
- 使用Sephadex LH-20列染色学净化COS.
- 使用UPLC-质谱学对COS进行量化和表征.
- 通过测量小鼠中的细胞因子水平 (IL-6,TNF-α,IFN-γ) 来评估免疫刺激活性.
- 通过测量小鼠的脚胀来评估抗炎活性.
主要成果:
- 纯化COS的特点是不同程度的聚合 (DP1-DP6) 和分子重量.
- 口服COSs显著增强了IL-6,TNF-α和IFN-γ在免疫抑制小鼠的胸腺和脏中的释放.
- 在500毫克每公斤-1.1的剂量下,COSs表现出显著的抗炎活性,与甲相当.
- 观察到的效果是在安全的剂量水平下实现的.
结论:
- 来自Bacillus cereus的生物制造的基托寡糖 (COS) 显示出有前途的免疫刺激和抗炎活性.
- COSs有效地刺激关键细胞因子标记物的产生,增强免疫反应.
- 这些发现表明COS是免疫调节和炎症管理的潜在治疗剂.
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