使用丸精子的新鲜和冷衍生性雌激体之间的明显的后授精动态
V Ferracuti1, A Bayram1,2, I Elkhatib1,3
1IVF Department, ART Fertility Clinics, Abu Dhabi, UAE.
Journal of assisted reproduction and genetics
|October 13, 2025
概括
使用新鲜的丸精子提取 (TESE) 精子与卵细胞最大限度地提高了受精率. 冷的精子或卵细胞增加了二核前 (2PN) 滞后时间,这表明冷后潜在的核修复.
科学领域:
- 生殖医学 生殖医学
- 不孕症研究 不孕症研究
- 精子冷保存 精子冷保存
背景情况:
- 丸精子提取 (TESE) 是一种在非阻塞性精子缺血 (NOA) 的情况下获取精子的方法.
- 在辅助生殖技术 (ART) 中使用冷解的精子和卵细胞是常见的,但它对早期后授精事件的影响需要评估.
研究的目的:
- 在使用TESE精子时,为了比较新鲜和冷衍生性雌激细胞之间的种植后结果.
- 为了研究新鲜与冷的丸精子和卵细胞对受精,胚胎细胞发育和euploidy率的影响.
主要方法:
- 一项回顾性队列研究分析了72个周期,涉及30对接受TESE的夫妇的980个卵子.
- 结合体包括来自同一患者的新鲜或冷解的TESE精子和新鲜或玻璃化卵子.
- 胚胎在时间缩短化器中进行培养,胚胎囊经过体皮活检进行植入前遗传测试,以检测体积 (PGT-A).
主要成果:
- 与新鲜TESE精子相比,冷TESE精子与明显较低的受精和爆发率有关.
- 每个被测试的胚胎囊的欧体积率在新鲜和冷TESE精子中是相似的,但被冷卵细胞使用没有显著影响.
- 冷TESE精子和玻璃化卵子都显示了显著更长的二核 (2PN) 到四细胞 (PNf) 阶段持续时间,这表明前核发育延迟.
结论:
- 同步新鲜的TESE精子采集与卵细胞采集可能会优化受精结果.
- 观察到的2PN发育与冷性子的延迟表明了潜在的解后核修复机制,这需要进一步研究.
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