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形态动力学参数S2预测了早期胚胎的发育潜力和相关的代谢途径
Xuehua Chen1, Huanhua Chen2, Guiting Huang2
1Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China.
Journal of assisted reproduction and genetics
|October 13, 2025
概括
测量早期胚胎细胞分裂时间的S2形态动力学参数适度地预测胚胎细胞形成和质量. 它的最佳切断时间为0.7小时,有助于评估胚胎发育潜力.
科学领域:
- 生殖医学 生殖医学
- 胚胎学 胚胎学
- 代谢学 代谢学 代谢学
背景情况:
- 评估早期胚胎发育潜力对于成功的试管婴儿结果至关重要.
- 来自时间间隔成像的形态动力学参数为胚胎发育提供了洞察力.
- S2参数代表了三细胞到四细胞裂变的持续时间,因此需要进一步研究其预测价值.
研究的目的:
- 确定S2形态动力学参数对早期胚胎发育潜力的预测值.
- 为了确定S2参数的最佳切割值.
- 探索与S2值和胚胎发育相关的代谢途径.
主要方法:
- 从65名患者的662个胚胎的前性分析,使用时间缩短化器.
- 接收器操作特征 (ROC) 分析,以确定最佳的S2截止值,以预测胚胎细胞形成和质量.
- 消耗的培养基 (SCM) 的新陈代谢概况使用液体染色学-并联质谱法 (LC-MS) 通过S2切断层分层.
主要成果:
- 该S2参数显示了对胚胎细胞形成 (AUC=0.622) 和质量 (AUC=0.645) 的适度预测能力.
- 确定了0.7小时的最佳S2截止时间,在胚胎细胞形成/质量组之间观察到显著差异.
- 代谢分析揭示了与蛋白质消化,氨基酸-tRNA生物合成和脂肪酸代谢相关的98种差异表达的代谢物.
结论:
- S2参数 (截止时间为0.7小时) 对胚胎发育潜力的预测值中等.
- 特定的代谢途径与S2调节有关,突出显示了形态动力学和代谢之间的联系.
- 该S2参数具有将其整合到多因素胚胎评估模型中的潜力.
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