通过双成像显微镜测量溶解体pH值
Emily Kirkham1, Sian Gardiner2, Emyr Lloyd-Evans1,2
1School of Biosciences, Cardiff University, Cardiff, UK.
Methods in molecular biology (Clifton, N.J.)
|October 13, 2025
概括
双波长比度光探测器可以定量测量亚细胞区内的离子含量. 本章详细介绍了使用这些探针测量溶酶体pH值的方法,包括选择,校准,仪器仪表和高通量测试小型化.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 双波长比度光探针对于量化亚细胞区内的离子含量至关重要.
- 溶解体pH值是影响细胞过程和疾病状态的关键参数.
研究的目的:
- 描述光探针用于精确的溶酶体pH测量的应用.
- 为使用这些技术的研究人员提供全面的指南.
主要方法:
- 选择合适的双波长比度光探针.
- 详细的校准程序用于准确的pH值测定.
- 用于光测量的仪器设置.
- 对于高通量选的小型化策略.
主要成果:
- 使用比率测量探针进行定量溶酶体pH测量的证明.
- 建立用于探头选择,校准和仪器仪表的协议.
- 对于高通量应用的试验成功微型化.
结论:
- 双波长比度光探针为定量亚细胞离子测量提供了黄金标准.
- 描述的方法有助于稳健和高通量溶酶体pH值的确定.
- 这个章节是细胞生物学和分析化学研究人员的宝贵资源.
相关概念视频
03:22Strong Acid and Base Solutions
A strong acid is a compound that dissociates completely in an aqueous solution and produces a concentration of hydronium ions equal to the initial concentration of acid. For example, 0.20 M hydrobromic acid will dissociate completely in water and produces 0.20 M of hydronium ions and 0.20 M of bromide ions.
03:27Mixtures of Acids
The pH of a solution containing an acid can be determined using its acid dissociation constant and its initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending upon the relative strength of the acids and their dissociation constants.
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
02:28Titration Calculations: Strong Acid - Strong Base
Calculating pH for Titration Solutions: Strong Acid/Strong Base
A titration is carried out for 25.00 mL of 0.100 M HCl (strong acid) with 0.100 M of a strong base NaOH. The pH at different volumes of added base solution can be calculated as follows:
(a) Titrant volume = 0 mL. The solution pH is due to the acid ionization of HCl. Because this is a strong acid, the ionization is complete and the hydronium ion molarity is 0.100 M. The pH of the solution is then:
A titration is carried out for 25.00 mL of 0.100 M HCl (strong acid) with 0.100 M of a strong base NaOH. The pH at different volumes of added base solution can be calculated as follows:
(a) Titrant volume = 0 mL. The solution pH is due to the acid ionization of HCl. Because this is a strong acid, the ionization is complete and the hydronium ion molarity is 0.100 M. The pH of the solution is then:
01:19Mixtures of Acids
The pH of a solution containing an acid can be determined using its acid dissociation constant and initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending on the relative strength of the acids and their dissociation constants.
In a strong and weak acid mixture, the strong acid dissociates completely and becomes a source of almost all the hydronium ions present in the solution. In contrast, the weak acid shows...
In a strong and weak acid mixture, the strong acid dissociates completely and becomes a source of almost all the hydronium ions present in the solution. In contrast, the weak acid shows...