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Updated: Aug 2, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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用光热显微镜对活细胞中的溶解体进行无标签的成像

Jun Miyazaki1, Ryotaro Wagatsuma2

  • 1Faculty of Systems Engineering, Wakayama University, Wakayama, Japan. jmiya@wakayama-u.ac.jp.

Methods in molecular biology (Clifton, N.J.)
|October 13, 2025
PubMed
概括

光热显微镜可视化活细胞中的内源染料,而无需标记. 这种光学成像技术通过检测积累的物质,可以进行溶酶体观察和功能分析.

关键词:
自自是一种自的过程.没有标签的无标签.活细胞成像成像技术光学吸收的光学吸收.光热显微镜 光热显微镜

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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.