在接种瑞生物科技mRNA疫苗BNT162b2后,T细胞和B细胞长度召回反应
Youmna El-Orfali1, Rana Mansour2, Habib Al-Kalamouni2
1Department of Experimental Pathology, Immunology, and Microbiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon; Department of Biological Sciences, Beirut Arab University, Beirut, Lebanon.
瑞生物科技的mRNA疫苗 (BNT162b2) 产生长期的免疫记忆. 尖端特异性T和B细胞在暴露于抗原时被重新激活,至少为9个月提供对SARS-CoV-2的保护.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 病毒学 病毒学
背景情况:
- 由SARS-CoV-2引起的COVID-19大流行导致全球数百万人死亡.
- 瑞生物科技mRNA疫苗 (BNT162b2) 诱导抗原特异性T和B细胞反应,与保护相关.
- 这些疫苗诱导细胞产生召回反应的能力以前没有被解决.
研究的目的:
- 为了确定BNT162b2接种疫苗后T细胞和B细胞召回反应的寿命,强度和表型.
- 分析BNT162b2疫苗产生的免疫记忆的特征.
主要方法:
- 12名参与者的血液样本在接种疫苗前,接种初级剂量后,以及在多个时间点收集到9个月后再接种剂量.
- 活体刺激抗原特异性B细胞和T细胞以分析它们的丰度,寿命和表型.
- 测量免疫球蛋白水平,动力学和中和潜力从血和激活的B细胞超浮体.
主要成果:
- 接种BNT162b2疫苗诱导了持久的中和免疫球蛋白和抗原特异性记忆CD4+T细胞.
- 记忆CD4+T细胞促进了记忆B细胞中的TH1反应和IgG/IgA类切换.
- 记忆 CD8+ T 细胞在重新激活时增殖,高调格兰酶和穿孔素以消除感染细胞.
结论:
- 接种BNT162b2疫苗可以建立尖端特异性记忆T细胞和B细胞.
- 这些记忆细胞在暴露于抗原时产生召回反应,从而提供对SARS-CoV-2的保护.
- 免疫保护在免疫接种后持续长达9个月.
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