在移植相关的缺血-再输液损伤中,将自与内皮细胞免疫性联系起来
Meredith E Taylor1, Dinesh Jaishankar1, Krishnaraju Madavaraju1
1Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA; Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Transplant immunology
|October 13, 2025
概括
自在调节心脏内皮细胞在冷和心脏移植后再注射损伤期间的免疫反应中发挥着关键作用. 增强自可能会保护捐赠心脏免受免疫攻击.
科学领域:
- 心血管科学 心血管科学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 心脏移植 (HTx) 涉及冷 (CS) 和缺血-再输液损伤 (IRI),这些损伤会损害供体心脏内皮细胞 (EC).
- EC通过自,通过细胞过程来适应CS / IRI压力,这对于恒常状态至关重要.
- 自在HTx期间EC激活和免疫性中的作用尚不清楚.
研究的目的:
- 在模拟的HTx条件下调查自在微血管心脏内皮细胞 (MCEC) 激活和免疫性中的作用.
- 探索调节自的潜力,作为减轻MCEC免疫性的一种预先条件策略.
主要方法:
- 在小鼠MCEC中使用遗传 (Atg5淘汰) 和药理学 (拉帕米辛,) 方法调节自.
- 在MCEC中,使用模拟HTx条件的冷储热再注射 (CS-WR) 模型对MCEC进行了测试.
- 评估了MCEC激活,细胞因子分泌和T细胞激活;从现有数据集中分析了人类EC自.
主要成果:
- 在CS期间减少MCEC的自,增加了它们的激活和免疫性.
- 用拉巴胺素药理诱导自,特别是在CS期间通过纳米载体,显著降低了MCEC免疫性.
- 在冷保存心脏中的人类EC显示了与自相关的基因表达的减少,支持了体外发现.
结论:
- 自在HTx的CS和IRI阶段对调节MCEC免疫性起着至关重要的作用.
- 在CS期间用自增强纳米载体预先调配MCEC,为减少捐赠心脏免疫性提供了一个有希望的策略.
更多相关视频
07:05Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy
Published on: September 27, 2024
832
09:25An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
Published on: May 8, 2020
8.3K
相关概念视频
01:40Cell-mediated Immune Responses
83.5K
Overview
83.5K
01:18Autophagic Cell Death
4.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.3K
01:27Autophagy
5.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.6K
01:24Regulation of Angiogenesis and Blood Supply
3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K