颗粒状后皮质的独特的转录基因细胞类型在小鼠和老鼠中被保存,尽管关键标记基因发生了剧烈变化
Isla A W Brooks1, Izabela Jedrasiak-Cape1, Chloe Rybicki-Kler1,2
1Department of Psychology, University of Michigan, Ann Arbor, Michigan 48109.
概括
鼠标和老鼠颗粒状后皮层 (RSG) 分享独特的细胞类型,对记忆和恐惧至关重要. 尽管进化保护,但特定物种的标记基因需要仔细考虑跨物种研究.
科学领域:
- 神经科学是一个神经科学.
- 比较解剖学的比较解剖学
- 分子生物学分子生物学
背景情况:
- 颗粒状后皮质 (RSG) 对于记忆,定向和恐惧处理至关重要.
- 鼠标RSG表现出独特的细胞类型,包括2/3层低基 (L2/3 LR) 和5a层金字塔 (L5a RSG) 神经元.
- 在小鼠和老鼠之间,这些独特的RSG细胞类型的进化保存是未知的.
研究的目的:
- 研究小鼠和老鼠之间的颗粒状后皮质 (RSG) 中的转录组独特细胞类型的进化保存.
- 确定跨物种标记基因,以准小鼠和大鼠的特定RSG细胞类型.
主要方法:
- 在小鼠和老鼠中,对粒状后皮层 (RSG) 细胞类型的比较转录基因分析.
- 跨物种的细胞类型特定标记基因的识别和验证.
主要成果:
- 鼠和老鼠RSG共享相同的独特细胞类型,占RSG神经元的50%以上.
- 关键标记基因,如老鼠L5aRSG神经元的Scnn1a,在老鼠中没有保存.
- 确定了替代的跨物种标记基因,用于针对两种物种中的RSG细胞类型.
结论:
- 颗粒状后皮质 (RSG) 独特的细胞类型在小鼠和老鼠之间在进化过程中得到保护.
- 标志性基因表达的显著物种特异性差异需要对跨物种遗传研究进行仔细选择.
- 已识别的标记物有助于开发特定于细胞类型的基因工具,用于小鼠和大鼠的RSG研究.
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