储存温度和离心前时间对新生日本黑色小牛的凝血参数的影响
Riko Ito1,2, Keinosuke Sanada2, Satoshi Inoue1
1Graduate School of Veterinary Sciences, Iwate University, Iwate, Japan.
The Journal of veterinary medical science
|October 13, 2025
概括
储存条件显著影响年轻小牛的血液检测结果. 为了准确的凝血和纤维解析测量,在采集后9小时内处理血液样本,以尽量减少变异.
科学领域:
- 兽医血液学 兽医血液学
- 凝血和纤维解析研究研究
- 动物生理学 动物生理学
背景情况:
- 准确的凝血和纤维溶解测试对于诊断和管理动物的静血性疾病至关重要.
- 预分析变量,包括样品储存,可以显著影响实验室测试结果.
- 关于离心前储存对年轻动物,特别是日本黑色小牛的凝血参数的影响的数据有限.
研究的目的:
- 研究离心前储存时间和温度对关键凝血和纤维解析标记物的影响.
- 为了建立最佳的分析前处理程序,从日本黑牛的血液样本.
主要方法:
- 在酸盐管中从日本黑色小牛 (6周以下) 采集了血液样本.
- 在离心之前,全血样本在室温或冷藏中存储了不同时间.
- 测量包括前热血素时间 (PT),激活的部分血栓形成时间 (aPTT),纤维素素和抗热血素水平.
主要成果:
- 当样品在采集后9小时内离心时,与在1小时内处理的样品相比,等离子体参数的变化不到10%.
- 室温和冷藏储存条件都在9小时窗口内显示了可接受的测试参数结果.
结论:
- 在室温或冷藏下储存全血的离心前存储时间长达9小时,不会显著改变日本黑色小牛的凝血和纤维素分解参数.
- 这一发现支持样品处理时间表的灵活性,允许采集后9小时的窗口进行离心,而不会影响PT,aPTT,纤维素素和抗血素测定在这个人群中的可靠性.
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