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微生物结合模块采用复杂的聚类糖贴片来选择性地附着在粘膜上

Thapakorn Jaroentomeechai¹, Billy Veloz², Cátia O Soares^3,4

¹Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.

Nature communications

|October 13, 2025

概括

微生物群使用粘素结合模块 (X409) 来识别粘液上的特定O-甘氨酸结构. 这种结合机制允许微生物粘附在粘液中,即使它们消耗了它的糖.

科学领域:

微生物学微生物学
结构生物学结构生物学
葡萄糖生物学葡萄糖生物学

背景情况:

湿体表面上的粘液层作为居住微生物群的关键屏障和接口.
富含O-甘氨酸的真蛋白通过捕捉,养和抑制毒性来塑造微生物群体.
微生物群与粘膜相互作用并降解粘膜的机制尚不清楚.

研究的目的:

阐明微生物群识别和与粘膜结合的分子机制.
为了识别介导微生物粘附的粘膜上特定的甘氨酸结构.

主要方法:

使用结构和分子分析来描述粘氨酸结合模块 (X409) 和粘氨酸糖之间的相互作用.
调查的重点是识别特定的O-glycan安排.

主要成果:

一个小的粘蛋白结合模块,X409,被发现可以识别相邻的O-糖中的聚合糖斑.
这些斑块由粘膜独特的内单糖排列组成.
结合这些聚类贴片可以确保微生物粘附于粘膜,即使O-甘氨酸被部分降解.

结论:

发现聚合的糖贴片结合动机揭示了微生物-素相互作用的新机制.
这一发现凸显了背景糖甘表位在微生物粘附中的重要性.
它为识别参与宿主微生物相互作用的新类甘氨酸结合蛋白提供了基础.

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