在人类血小板中,TLR2诱导的表面动员和CD14的释放
Anna Kobsar1, Daniela Simao Vaz1, Julia Zeller-Hahn1
1Clinical Transfusion Medicine and Haemotherapy, University Clinic of Wuerzburg, Oberduerrbacher Straße 6, 97080, Wuerzburg, Germany.
Scientific reports
|October 14, 2025
概括
人类血小板含有CD14,这是通用类受体 (TLR) 的共同受体. 血小板在受到TLR2激动剂或原刺激时表达CD14,并在TLR2激活后释放可溶性CD14 (sCD14).
科学领域:
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
背景情况:
- 血小板除了血液静止之外,还参与免疫反应.
- 血小板表达类似收费受体 (TLR),如TLR2和TLR4.
- CD14是免疫细胞中TLR信号传递的关键共受体.
研究的目的:
- 研究人体血小板中CD14的存在,释放和分布.
- 了解CD14在血小板免疫功能中的作用.
- 检查与TLRs相关的CD14表达和定位.
主要方法:
- 使用流式细胞计,免疫检测,西布局,传输电子显微镜和光显微镜对洗净的人类血小板 (WP) 进行分析.
- 用TLR2激动剂 (Pam3CSK4) 和TLR4激动剂 (LPS) 刺激血小板.
- 检测CD14表达,溶性CD14 (sCD14) 和细胞内CD14定位.
主要成果:
- 通过TLR2激动剂或原激发的血小板上检测到CD14表达,但在静止或TLR4激发的血小板中没有检测到.
- TLR2的激活触发了sCD14的释放.
- 细胞内CD14被观察到沿着血小板膜,与TLR2和TLR4共同定位.
结论:
- 人类血小板含有CD14分子.
- 血小板CD14可以被招募到表面,并在特定刺激时释放为sCD14.
- 血小板中的CD14与TLR2和TLR4共同局部化,这表明它在TLR介导的血小板激活中发挥了作用.
相关概念视频
01:22Formation of the Platelet Plug
8.7K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
8.7K
01:18Structure and Function of Platelets
2.9K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
2.9K