相关实验视频

Updated: Jan 15, 2026

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Published on: May 12, 2017

溶酶体应激反应和线粒体损伤是FSGS中最早可检测到的变化

Eileen Dahlke¹, Jessica Promnitz¹, Mairon Trujillo Miranda¹

¹Institute of Anatomy, Christian-Albrechts-University Kiel, Otto-Hahn-Platz 8, 24118, Kiel, Germany.

Scientific reports

|October 14, 2025

概括

lysosome 压力和线粒体损伤是焦点细分结核硬化 (FSGS) 的早期事件. 这些细胞功能障碍可能引发炎症,导致细胞损伤和疾病进展.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
细胞生物学细胞生物学
病理学病理学病理学

背景情况:

焦点细分型淋巴细胞硬化 (FSGS) 涉及细胞功能障碍和损失.
细胞需要溶解体和线粒体的完整性和代谢适应.
在FSGS病变发生过程中,溶酶体和线粒体参与的时间序列尚不清楚.

研究的目的:

调查FSGS中致病事件的时间依赖序列.
阐明 lysosomes 和线粒体在 FSGS 发育中的早期作用.

主要方法:

使用一种可诱导的小鼠模型,具有 podocyte 特定的 Nphs2 删除.
在FSGS诱导后的5,9,17天分析了球病理.

主要成果:

最早的变化 (5天) 包括溶酶体应激,线粒体损伤和增加的CXCL1.
在9天后,观察到间血管增殖和 lysosome/mitochondria应激增加.
严重的伤害,足细胞平坦化,硬化和ER压力在17天后就显而易见.

结论:

溶酶体应激和线粒体损伤是FSGS中最早可检测到的事件.
这些器官可以通过信号通路和化学激素的作用启动炎症.
细胞内膜网膜应激似乎延续了细胞损伤和FSGS.

关键词:

这是FSGS,FSGS.lysosome 应激酶的压力是什么线粒体损伤的损伤细胞是一种细胞.

更多相关视频

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Published on: July 3, 2017

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

相关概念视频

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...