斯基索菲勒姆群落EO22的细胞酶活动增加,与链球菌素二元联结
I G Shirokikh1,2, N A Bokov3,4, A A Shirokikh5,6
1Rudnitsky Federal Agricultural Research Center of the Northeast, Kirov, Russia. irgenal@mail.ru.
概括
与 Schizophyllum commune (S. commune) 建立细菌-真菌关联,加速细胞酶活动,以有效地生物转化谷物草. 这些新型微生物联盟促进了草的分解,为农业废物利用提供了一个有前途的战略.
科学领域:
- 生物技术和微生物生态学
- 农业废物的生物转化.
- 酶活动优化酶活动优化
背景情况:
- 石菌 (Schizophyllum commune,S. commune) 是一种多功能白色腐烂真菌,在农业废物生物转化,特别是谷物中应用有限.
- 通过共同培养来激活和控制纤维素降解生物中的酶活性是一个重大挑战.
- 有效利用像草这样的作物生产副产品需要创新的生物技术方法.
研究的目的:
- 评估使用S. commune EO22和Streptomyces细菌创造人工细菌-真菌协会的可行性.
- 通过增强微生物联盟,制定利用谷物作为基质的有效策略.
- 调查S. commune与特定的纤维溶解细菌共同培养对细胞酶活性和草分解的影响.
主要方法:
- 在液态矿物质介质中与四个菌株的Streptomyces细菌 (Mb4-2,T1-3,N27-25,H13-3) 共同培育S.commune EO22与草作为唯一的碳来源.
- 随着时间的推移,监测单种和二元培养中细胞酶活性的动态.
- 通过测量体重减轻来量化吸管分解.
主要成果:
- 二元培养物比S. commune单种培养物早3-6天达到最大的细胞酶活性.
- 与Streptomyces菌株T1-3,N27-25和H13-3共同培养导致最大细胞酶活性分别增加2.3倍,1.6倍和1.3倍.
- 与真菌单种作物相比,的分解分别增加了10.3%,2.3%和22.4%的菌株Mb4-2,T1-3和N27-25,尽管与细胞酶活性没有显著相关.
结论:
- 人工细菌和真菌的结合,代表了一种有前途的方法,可以有效地降解谷物和其他纤维素废物.
- 共同培养策略可以显著提高S. commune的酶能力,用于生物转化应用.
- 对优化这些微生物联盟的进一步研究可能会为可持续的废物管理和资源利用开辟新的途径.
相关概念视频
01:28Fungal Phylum Ascomycota
1.0K
Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
1.0K
01:29Fungal Group Zygomycota
995
Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
995
01:02Role of Microtubules in Cell Wall Deposition
3.0K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
3.0K