从慢性感染患者中复发的Mycobacterium avium分离体的基因组表征揭示了宿主内部进化的模式
Aaron M Walsh1,2, Emma Roycroft3,4, Kate Hinchion5
1School of Medicine, Trinity Translational Medicine Institute, St James's Hospital, Trinity College Dublin, Dublin, Ireland. awalsh12@tcd.ie.
Genome medicine
|October 14, 2025
概括
整个基因组测序揭示了Mycobacterium avium复合体在患者内演变,通过基因选择获得新菌株和适应. 这种适应可能会增强耐药性和抗生素耐药性,提供新的治疗点.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 菌复合体 (MAC) 导致持续性,难以治疗的感染,特别是在脆弱人群中.
- 全球MAC感染的发病率不断增加,需要了解其持续机制.
- 全基因组测序 (WGS) 提供了一种强大的工具来研究MAC在人类肺部的长期演变.
研究的目的:
- 通过使用全基因组测序来分析Mycobacterium avium复合物的宿主内部进化.
- 将来自爱尔兰的MAC菌株与国际队列进行比较,以识别流通的克隆.
- 调查MAC在慢性感染期间使用的适应策略.
主要方法:
- 分析了来自56名患者的287个M. avium分离物,其中包括来自10年爱尔兰队列的50个新测序的基因组.
- 与来自欧洲群体的237个已发表的基因组进行比较,以将爱尔兰菌株置于背景.
- 在三个队伍中进行组合分析,以追踪感染期间的变化.
主要成果:
- 识别了M. avium菌株的国际集群,表明全球占主导地位的克隆.
- 在宿主中观察到M. avium群落的演变,并经常获得新的菌株.
- 发现了13个真菌细菌基因中积极选择的证据,包括毒性和抗生素耐药性基因.
结论:
- WGS为M. avium.宿主体内的进化提供了新的见解.
- 确定了潜在的真菌细菌适应策略,以提高宿主持久性.
- 突出了M. avium感染的潜在新治疗点.
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