[帕纳克斯人参中的B3基因家族的鉴定和表达分析]
Yu-Long Wang1, Ai-Min Wang1, Jing-Hui Yu1
1College of Life Science, Jilin Agricultural University Changchun 130118, China Research Center for Ginseng Genetic Resources Development and Utilization, Jilin Province,Jilin Agricultural University Changchun 130118, China.
概括
研究人员在Panax人参中鉴定出145个B3基因,发现15个与人参化物产生和应激反应有关. 这项研究为了解P.人参提供了基础.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 潘纳克斯人参是一种有价值的药用草药,具有多样化的药理性质.
- B3基因家族对植物生长,发育和应激弹性至关重要.
- 之前没有任何研究确定或分析了P.人参中的B3基因家族.
研究的目的:
- 为了识别和分析Panax人参中的B3基因家族.
- 为了研究PgB3基因在人参化物生物合成中的作用.
- 探索PgB3基因在应激反应中的参与.
主要方法:
- 在P. ginseng中全基因组识别B3基因.
- 根据域分析,将已识别的B3基因分类为子家族.
- 与人参化物含量的相关性分析和SNP/InDel分析.
- 基因相互作用网络分析与人参酸生物合成中的关键酶.
- 在甲基莉酸盐 (MeJA) 应激下对基因表达的分析.
主要成果:
- 总共有145个B3基因 (PgB3s) 被确定并分为五个子家族.
- 十五个PgB3转录显示与人参化物含量有显著相关性.
- 这些15个PGB3s形成一个密切的相互作用网络与关键的酶基因在人参酸生物合成.
- 在MeJA压力下,PgB3基因表现出诱导的表达,这表明它在抗压力方面发挥了作用.
- 促成体分析显示,在PgB3基因中,有大量的应激反应元素.
结论:
- 已识别的PgB3基因可能参与调节P. ginseng. ginseng中的金氏生物合成.
- B3基因家族在P.人参对压力的反应中起着重要作用.
- 这项研究为进一步研究PgB3基因功能和调控机制提供了理论基础.
更多相关视频
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
10.9K
07:34Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
4.9K
相关概念视频
01:58Cell Specific Gene Expression
16.2K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.2K
01:25Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K