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在贫血糖尿病足的LncRNA和mRNA表达特征和生物信息分析

Jiayu Lin¹, Jingying Wang¹, Bo Liang¹

¹Department of Endocrinology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.

Frontiers in genetics

|October 14, 2025

概括

这项研究揭示了糖尿病足 (DFU) 中的新型长非编码RNA (lncRNA) -信使RNA (mRNA) 网络与血红蛋白缺乏相关. 这些网络突出突出破坏铁代谢和炎症,为贫血的DFU提供潜在的诊断标记.

科学领域:

生物化学生化学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

糖尿病足 (DFU) 是一种严重的并发症,通常与血红蛋白缺乏有关.
转录基因变化与DFU的发病有关,但长非编码RNAs (lncRNAs) 在将血红蛋白异常与铁炎症失调联系中的作用尚不清楚.

研究的目的:

确定与铁代谢和炎症相关的DFU特定的lncRNA-mRNA联合表达网络.
评估这些网络在DFU患者中的诊断潜力.

主要方法:

在DFU患者和对照患者的皮肤组织上进行了RNA测序.
使用DESeq2.2识别了差异表达的lncRNA和mRNA.
用基因组丰富分析 (GSEA) 和共同表达网络构建来分析功能途径和相互作用.

主要成果:

在DFU组织中发现了21种差异表达的lncRNA和368种差异表达的mRNA.
GSEA揭示了与血红蛋白相关的途径的显著丰富,包括铁代谢,氧化应激和炎症.
一个共同表达网络绘制了关键的lncRNAs和mRNAs之间的相互作用,这些相互作用与铁失调和慢性炎症有关.

结论:

在DFU中发现了与血红蛋白缺乏相关的明显的lncRNA-mRNA联合表达网络.
关键的lncRNA与参与铁处理和炎症的mRNA有很强的相关性,这表明它们对DFU病理的贡献.
这些发现突出了lncRNAs在贫血的DFU中的潜在调节作用,并为诊断开发提供了途径.

关键词:

贫血贫血是一种疾病.生物信息分析分析生物信息分析慢性炎症是一种慢性炎症.糖尿病人的足部.铁代谢失调铁代谢失调长长的非编码RNAs.

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...