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用DNA架构的双特异性抗体增强中细胞干细胞治疗炎症性肠病的治疗方法

Xi Liu¹, Tenghui Ye¹, Leyi Yang¹

¹School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, P. R. China.

Advanced materials (Deerfield Beach, Fla.)

|October 14, 2025

概括

在炎症性肠病 (IBD) 模型中,带有双特异性抗体的工程化介质细胞 (MSCs) 显示了对炎症结肠的改善指导. 这通过减少白细胞透和促进粘膜修复来增强治疗效果.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

介酶体 stromal 细胞 (MSCs) 由于其免疫调节和再生性质,显示出对炎症性肠病 (IBD) 的治疗潜力.
目前对IBD的MSC治疗受限于对炎症部位的不良指导和过度的白细胞透.

研究的目的:

设计MSC以增强向和减少白细胞招募用于IBD治疗.
为MSCs开发基于DNA纳米技术的双特异抗体修饰策略.

主要方法:

使用DNA纳米技术,MSCs被修改为Y形双特异抗体 (YMV),集成抗VCAM-1和抗MAdCAM-1抗体.
评估了粘附效率和白细胞粘附抑制.
在IBD的小鼠模型中评估了YMV修饰的MSCs (YMV-MSCs).

主要成果:

与原生MSC相比,YMV修改的MSC的粘合效率增加了三倍.
YMV-MSCs有效地竞争MAdCAM-1结合部位,并显著抑制白细胞粘附.
在IBD小鼠模型中,YMV-MSCs表现出增强的结肠回归,减少白细胞透和减弱炎症.

结论:

通过DNA介导的双特异性抗体修饰增强了MSC针对炎症组织的向.
这一策略通过阻断白细胞招募和促进粘膜修复来提高MSC在IBD的治疗疗效.
YMV-MSC平台为先进的基于细胞的IBD治疗提供了一个有前途的方法.

关键词:

自组装DNA的自我组装.两种特异性抗体的抗体细胞表面工程细胞表面工程炎症性肠病是一种炎症性肠病.介质细胞层层细胞.

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