在Streptococcus pneumoniae引起的肺炎期间,肺部营养可用性的变化
Hansol Im1,2, Vipin Chembilikandy1, Adonis D'Mello3
1Department of Microbiology, Heersink School of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
这项研究揭示了Streptococcus pneumoniae感染如何在肺炎期间改变肺部代谢. 细菌基因表达,特别是spxB,随着疾病阶段的显著变化,影响营养的可用性和宿主-病原体相互作用.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 宿主-病原体相互作用
背景情况:
- 肺炎链球菌是导致肺炎的主要原因之一.
- 肺炎对呼吸道代谢和细菌基因表达的影响尚不清楚.
研究的目的:
- 在肺炎球菌性肺炎期间描述呼吸道的代谢场景.
- 研究宿主代谢变化对细菌基因表达和表型的影响.
主要方法:
- 未定位的UPLC-ESI-MS/MS代谢组学,以分析气道代谢物.
- 在NanoString nCounter中分析细菌基因表达 (66个基因).
- 主体代谢转变与细菌转录变化的相关性.
主要成果:
- 在肺炎各个阶段观察到气道代谢物的动态变化,最初的增加随后下降.
- 主体代谢干扰表明 purin 失调,细胞应激和组织损伤.
- 随着肺炎的进展,葡萄糖水平下降,与细菌中spxB基因表达的增加相关,这表明转向混合发酵和过氧化生产.
结论:
- 肺炎球菌性肺炎会在呼吸道中引起重大宿主代谢变化.
- 这些代谢变化会影响细菌的适应,包括毒性和应激反应.
- 了解这些宿主-病原体的代谢动态可以为肺炎的新疗法策略提供信息.
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