分析电流对细胞膜完整性的破坏作用,并对其抗菌活性进行建模
Zeynep Gergin1, Fatih Tarlak2, Bengü Erguden3
1Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey.
Archives of microbiology
|October 14, 2025
概括
电刺激 (ES) 通过破坏细胞膜,有效抑制细菌生长. 机器学习模型预测了抑制区,有助于杀菌和感染控制应用.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 计算生物学 计算生物学
背景情况:
- 抗微生物耐药性需要新的灭菌方法.
- 电刺激 (ES) 显示出作为化学剂的替代品的希望.
- 优化ES参数和开发预测工具至关重要.
研究的目的:
- 为了研究ES对格拉姆阴性和格拉姆阳性细菌的抗菌疗效.
- 探索ES对细菌细胞膜完整性的影响.
- 使用机器学习开发ES抗菌活性的预测模型.
主要方法:
- 对大肠杆菌,P. aeruginosa,S. aureus和S. epidermidis暴露于不同的ES电压和持续时间.
- 对细菌生长抑制区的评估.
- 在ES后对细菌细胞膜完整性的分析.
- 机器学习回归 (SVR,RF,GPR) 用于模拟抑制区.
主要成果:
- ES显著抑制了所有测试的细菌菌株.
- 在阴极和阳极观察到明显的抑制区.
- 治疗ES破坏了大肠杆菌和黄金杆菌的细胞膜完整性.
- 高斯过程回归 (GPR) 成为最好的预测模型.
结论:
- 电刺激是一种强大的抗微生物剂.
- ES破坏了细菌细胞膜的完整性,导致生长抑制.
- 基于GPR的预测工具被开发用于ES在绝育和感染管理中的应用.
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