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在黎巴嫩队列中SARS-CoV-2入口基因表达的时空空间和人口结构模式

Fatima Al Nemer¹, Mohammad Fayyad-Kazan², Bassam Badran¹

¹Laboratory of Cancer Biology and Molecular Immunology, Faculty of Science I, Lebanese University, Hadath-Beirut, Lebanon.

Molecular biology reports

|October 14, 2025

概括

鼻子中的SARS-CoV-2入口基因表达因年龄和性别而异. ACE2,FURIN和CTSL水平随着年龄的增长而下降,但女性的水平较高,可能会影响COVID-19的易感性和保护.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

在鼻膜上,SARS-CoV-2利用宿主进入因子,如ACE2,TMPRSS2,FURIN和CTSL.
了解这些基因表达的人口和感染阶段变异对于了解COVID-19易感性和进展至关重要.

研究的目的:

在健康的黎巴嫩人群中评估SARS-CoV-2宿主入口基因 (ACE2,TMPRSS2,FURIN,CTSL) 的基线鼻表达.
根据年龄,性别和吸烟情况来分层表达.
为了研究这些基因在轻度COVID-19感染期间的动态调节.

主要方法:

在173名COVID-19阴性个体中进行鼻基因表达分析 (ACE2,TMPRSS2,FURIN,CTSL).
用RT-qPCR对四个感染阶段的84例轻度COVID-19病例进行了长度研究.
促进者分析以确定监管转录因素.

主要成果:

基线ACE2,FURIN和CTSL的表达随着年龄的增长而下降;TMPRSS2显示出双相模式.
女性的ACE2,FURIN和CTSL表达率高于男性;吸烟显示上调的趋势不显著.
在感染期间,ACE2,TMPRSS2和FURIN表现出钟形的表达模式,而CTSL保持稳定.

结论:

鼻入口基因表达受到年龄,性别和感染阶段的显著影响.
在儿童和女性中,基线表达较高可能有助于早期免疫反应和保护.
确定了转录因子结合部位,解释了观察到的表达模式,并突出了这些基因在COVID-19病变发生过程中的复杂作用.

关键词:

进入基因进入基因鼻上皮的鼻上皮.病变的发生和发病.这就是SARS-CoV-2病毒.

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