有高线粒体RNA含量的纤维细胞和髓状细胞代表了OA同胞体内的生物学意义上的种群
Wesley Tran1, Garth Blackler1, Rebecca Luo1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada; Bone and Joint Institute, University of Western Ontario, London, ON, Canada.
Osteoarthritis and cartilage
|October 14, 2025
概括
骨关节炎 (OA) 中高线粒体RNA含量 (pMT) 细胞是可活的和与疾病相关的,不会受到压力或死亡. 排除这些细胞可能会掩盖关键的骨关节炎病理生物学,需要特定于环境的质量控制策略.
科学领域:
- 单细胞基因组学 单细胞基因组学
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
背景情况:
- 标准的单细胞RNA测序 (scRNA-seq) 质量控制 (QC) 通常会去除具有高线粒体RNA含量 (pMT) 的细胞.
- 这种排除通常基于这样的假设:高pMT细胞表明细胞活力不佳或解离过程中的压力.
- 然而,新出现的证据表明,高pMT细胞可能代表生物学上显著的细胞群.
研究的目的:
- 调查排除高pMT细胞对膝关节骨关节炎 (OA) 的转录景观的影响. synovium.
- 探索高pMT细胞在OA疾病病理生物学中的潜在作用.
主要方法:
- 重新分析了7个人类和小鼠scRNA-seq数据集,使用高pMT细胞的包容性QC值.
- 评估了pMT分布,解离诱导的压力,细胞死亡和线粒体亡基因签名.
- 在传统和高pMT综合管道之间,在人类膝关节OA突数据集 (GSE248453) 中比较差异性基因表达和通路丰富.
主要成果:
- 高pMT细胞与解离诱导的压力或亡途径没有相关性,表明活力.
- 高pMT细胞主要在纤维细胞和髓质子集中发现.
- 高pMT的突纤维细胞被丰富了细胞外矩阵 (ECM) 重塑路径.
- 高pMT髓质细胞与炎症信号和免疫激活有关.
结论:
- 在OA同胞体中的高pMT细胞是可活的,并且可能与疾病相关.
- 排除这些细胞可能会掩盖OA病理生理学的关键方面.
- 特定于背景的质量控制策略对于开放学科研究至关重要.
- 高pMT的纤维细胞和髓状细胞群体需要进一步研究作为潜在的OA驱动因素.
相关概念视频
Introduction to Fibroblasts
3.8K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.8K
Bone Cells and Tissue
7.8K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
7.8K
Formation of Muscle Fibers from Myoblasts
5.7K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.7K
T Cell Types and Functions
2.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.1K
Osteoclasts in Bone Remodeling
3.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.8K


