PFAS和微塑料混合物在五个人类细胞系中的协同毒性
Mia Sands1, Arshveer Sachdeva2, Laura Bukavina3
1Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL, 61801, USA; Biomedical Research Center, Mills Breast Cancer Institute, Carle Foundation Hospital, Urbana, IL, 61801, USA.
Environmental pollution (Barking, Essex : 1987)
|October 14, 2025
概括
和多基物质 (PFAS) 和微塑料 (MPs) 在人类和肝细胞中表现出协同毒性,增加氧化应激和DNA损伤. 混合物效应取决于剂量和比例,突出了对环境污染物的联合暴露的担忧.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 和多基物质 (PFAS) 和微塑料 (MPs) 是广泛存在的环境污染物.
- 同时暴露于PFAS和MP是常见的,但它们对健康的综合影响尚不清楚.
研究的目的:
- 评估单个和组合的PFAS (PFOA,GenX) 和MP (聚烯,聚乙烯) 暴露的细胞毒性,氧化和基因毒性影响.
- 为了研究人类 (A498) 和肝 (HepG2) 细胞系的差异反应.
主要方法:
- 五个人类细胞系 (A498,HepG2,PC3,A431,A549) 暴露在PFOA,GenX,聚烯和LDPE的环境相关度下.
- 对细胞毒性,活性氧物种 (ROS) 生产,抗氧化基因表达和DNA损伤/修复途径的评估.
主要成果:
- 协同毒性主要在和肝细胞中观察到,ROS增加,抗氧化基因表达升高和DNA修复激活.
- 在所有终点上,A498细胞的敏感性高于HepG2细胞.
- 混合物毒性取决于剂量和PFAS与MP的比率,主要是协同效应.
结论:
- 结合PFAS和MP暴露可以诱导人体细胞中的协同氧化应激和基因毒性,特别是在和肝脏中.
- 风险评估必须考虑混合物毒性,氧化应激和基因毒性作为关键机制.
- 不同的细胞反应凸显了同时暴露效应的复杂性.
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